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Product Info Summary
| SKU: | LS023625 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of family with sequence similarity 89, member B (FAM89B), transcript variant 1 |
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Product info
Product Name
FAM89B (NM_001098785) Human Over-expression Lysate
SKU/Catalog Number
LS023625
Size
100 µg
Description
Transient overexpression lysate of family with sequence similarity 89, member B (FAM89B), transcript variant 1
Storage & Handling
Cite This Product
FAM89B (NM_001098785) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023625)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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FAM89B Human Over-expression Lysates NM_001098784.
Western blot validation of overexpression lysate (LS023625) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FAM89B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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FAM89B Human Over-expression Lysates NM_001098785.
Western blot validation of overexpression lysate (LS023625) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FAM89B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
FAM89B Human Over-expression Lysates NM_152832.
Western blot validation of overexpression lysate (LS023625) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FAM89B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Fam89b (Source: Uniprot.org, NCBI)
Gene Name
Fam89b
Full Name
Leucine repeat adapter protein 25
Weight
20.14kDa
Superfamily
FAM89 family
Alternative Names
family with sequence similarity 89, member B; mammary tumor virus receptor homolog 1; MTVR1 Fam89b|1110021A21Rik, Fam89, MMTV, Mtv, Mtvr2|family with sequence similarity 89, member B|leucine repeat adapter protein 25|C184L ORF2 protein|C184M protein|MMTV receptor|mammary tumor virus receptor 2|protein FAM89B
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Fam89b, check out the Fam89b Infographic
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In this infographic, you will see the following information for Fam89b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For FAM89B (NM_001098785) Human Over-expression Lysate (LS023625)
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