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Product Info Summary
| SKU: | LS022862 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of fibronectin type III domain containing 3A (FNDC3A), transcript variant 2 |
Product info
Product Name
FNDC3A (NM_014923) Human Over-expression Lysate
SKU/Catalog Number
LS022862
Size
100 µg
Description
Transient overexpression lysate of fibronectin type III domain containing 3A (FNDC3A), transcript variant 2
Storage & Handling
Cite This Product
FNDC3A (NM_014923) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS022862)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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FNDC3A Human Over-expression Lysates NM_014923.
Western blot validation of overexpression lysate (LS022862) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FNDC3A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for FNDC3A (Source: Uniprot.org, NCBI)
Gene Name
FNDC3A
Full Name
Fibronectin type-III domain-containing protein 3A
Weight
131.852kDa
Superfamily
FNDC3 family
Alternative Names
bA203I16.1; bA203I16.5; fibronectin type III domain containing 3; fibronectin type III domain containing 3A; fibronectin type-III domain-containing protein 3A; FNDC3; HUGO; Human gene expressed in odontoblasts; KIAA0970FLJ31509 FNDC3A FNDC3, HUGO, bA203I16.1, bA203I16.5 fibronectin type III domain containing 3A fibronectin type-III domain-containing protein 3A|human gene expressed in odontoblasts
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on FNDC3A, check out the FNDC3A Infographic
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In this infographic, you will see the following information for FNDC3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For FNDC3A (NM_014923) Human Over-expression Lysate (LS022862)
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No publications found for LS022862
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