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Product Info Summary
| SKU: | LS055568 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10) (GALNT10), transcript variant 1 |
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Product info
Product Name
GALNT10 (NM_198321) Human Over-expression Lysate
See all Polypeptide GalNac Transferase 10/GALNT10 products
SKU/Catalog Number
LS055568
Size
100 µg
Description
Transient overexpression lysate of UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10) (GALNT10), transcript variant 1
Storage & Handling
Cite This Product
GALNT10 (NM_198321) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS055568)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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GALNT10 Human Over-expression Lysates NM_017540.
Western blot validation of overexpression lysate (LS055568) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with GALNT10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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GALNT10 Human Over-expression Lysates NM_198321.
Western blot validation of overexpression lysate (LS055568) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with GALNT10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for GALNT10 (Source: Uniprot.org, NCBI)
Gene Name
GALNT10
Full Name
Polypeptide N-acetylgalactosaminyltransferase 10
Weight
68.992kDa
Superfamily
glycosyltransferase 2 family
Alternative Names
DKFZp586H0623; EC 2.4.1.41; FLJ00205; FLJ11715; GalNAc transferase 10; GALNACT10; GalNAc-T10; GALNT10; Polypeptide GalNAc transferase 10; polypeptide N-acetylgalactosaminyltransferase 10; PPGALNACT10; pp-GaNTase 10; PPGANTASE10; Protein-UDP acetylgalactosaminyltransferase 10; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10; UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 10 (GalNAc-T10) GALNT10 GALNACT10, PPGALNACT10, PPGANTASE10 polypeptide N-acetylgalactosaminyltransferase 10 polypeptide N-acetylgalactosaminyltransferase 10|GalNAc transferase 10|UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10|UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on GALNT10, check out the GALNT10 Infographic
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In this infographic, you will see the following information for GALNT10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For GALNT10 (NM_198321) Human Over-expression Lysate (LS055568)
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