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Product Info Summary
| SKU: | LS009934 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1 |
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Product info
Product Name
GPR105 (P2RY14) (NM_001081455) Human Over-expression Lysate
SKU/Catalog Number
LS009934
Size
100 µg
Description
Transient overexpression lysate of purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1
Storage & Handling
Cite This Product
GPR105 (P2RY14) (NM_001081455) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009934)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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GPR105 (P2RY14) Human Over-expression Lysates NM_001081455.
Western blot validation of overexpression lysate (LS009934) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with P2RY14 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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GPR105 (P2RY14) Human Over-expression Lysates NM_014879.
Western blot validation of overexpression lysate (LS009934) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with P2RY14 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for P2RY14 (Source: Uniprot.org, NCBI)
Gene Name
P2RY14
Full Name
P2Y purinoceptor 14
Weight
38.971kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
G protein coupled receptor for UDP-glucose; GPR105; G-protein coupled receptor 105; KIAA0001G protein-coupled receptor 105; P2RY14; P2Y purinoceptor 14; P2Y(14) receptor; P2Y14 receptor; P2Y14; purinergic receptor P2Y, G-protein coupled, 14; UDP-glucose receptor P2RY14 BPR105, GPR105, P2Y14 purinergic receptor P2Y14 P2Y purinoceptor 14|G protein coupled receptor for UDP-glucose|G-protein coupled receptor 105|P2Y(14) receptor|P2Y14 receptor|UDP-glucose receptor|purinergic receptor P2Y, G-protein coupled, 14
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on P2RY14, check out the P2RY14 Infographic
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In this infographic, you will see the following information for P2RY14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For GPR105 (P2RY14) (NM_001081455) Human Over-expression Lysate (LS009934)
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