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Product Info Summary
| SKU: | LS344561 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of G protein-coupled receptor 148 (GPR148) |
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Product info
Product Name
GPR148 (NM_207364) Human Over-expression Lysate
SKU/Catalog Number
LS344561
Size
100 µg
Description
Transient overexpression lysate of G protein-coupled receptor 148 (GPR148)
Storage & Handling
Cite This Product
GPR148 (NM_207364) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS344561)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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GPR148 Human Over-expression Lysates NM_207364.
Western blot validation of overexpression lysate (LS344561) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with GPR148 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for GPR148 (Source: Uniprot.org, NCBI)
Gene Name
GPR148
Full Name
Probable G-protein coupled receptor 148
Weight
38.288kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Brain and testis restricted GPCR; BTR; G protein-coupled receptor 148; GPR148; G-protein coupled receptor PGR6; PGR6; PGR6G protein-coupled receptor PGR6; probable G-protein coupled receptor 148 GPR148 BTR, PGR6 G protein-coupled receptor 148 probable G-protein coupled receptor 148|G protein-coupled receptor PGR6|brain and testis restricted GPCR
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on GPR148, check out the GPR148 Infographic
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In this infographic, you will see the following information for GPR148: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For GPR148 (NM_207364) Human Over-expression Lysate (LS344561)
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No publications found for LS344561
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