HOXD8 (NM_019558) Human Over-expression Lysate

Lysate of HOXD8 over-expression cell lines, WB control

Transient overexpression lysate of homeobox D8 (HOXD8)

Product Info Summary

SKU: LS003234
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of homeobox D8 (HOXD8)

Product Name

HOXD8 (NM_019558) Human Over-expression Lysate

See all HOXD8 products

SKU/Catalog Number

LS003234

Size

100 µg

Description

Transient overexpression lysate of homeobox D8 (HOXD8)

Storage & Handling

Cite This Product

HOXD8 (NM_019558) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003234)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for HOXD8 (Source: Uniprot.org, NCBI)

Gene Name

HOXD8

Full Name

Homeobox protein Hox-D8

Weight

31.911kDa

Superfamily

Antp homeobox family

Alternative Names

homeo box 4E; homeo box D8; homeobox D8; homeobox protein 5.4; Homeobox protein Hox-4E; Homeobox protein Hox-5.4; homeobox protein Hox-D8; HOX4; HOX4EHox-4.5; HOX5.4 HOXD8 HOX4, HOX4E, HOX5.4 homeobox D8 homeobox protein Hox-D8|Hox-4.5|homeo box 4E|homeo box D8|homeobox protein 5.4|homeobox protein Hox-4E|homeobox protein Hox-5.4

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on HOXD8, check out the HOXD8 Infographic

HOXD8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HOXD8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for LS003234

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used HOXD8 (NM_019558) Human Over-expression Lysate?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

Be the first to review HOXD8 (NM_019558) Human Over-expression Lysate

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for HOXD8 (NM_019558) Human Over-expression Lysate

$628
Backordered.

Lead time for this item is typically 2 weeks

Get A Quote
In stock
Order Product
LS003234
$628.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.