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Product Info Summary
| SKU: | LS051171 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of hydroxysteroid (17-beta) dehydrogenase 14 (HSD17B14) |
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Product info
Product Name
HSD17B14 (NM_016246) Human Over-expression Lysate
See all 17 beta-HSD14/HSD17B14 products
SKU/Catalog Number
LS051171
Size
100 µg
Description
Transient overexpression lysate of hydroxysteroid (17-beta) dehydrogenase 14 (HSD17B14)
Storage & Handling
Cite This Product
HSD17B14 (NM_016246) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS051171)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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HSD17B14 Human Over-expression Lysates NM_016246.
Western blot validation of overexpression lysate (LS051171) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with HSD17B14 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for HSD17B14 (Source: Uniprot.org, NCBI)
Gene Name
HSD17B14
Full Name
17-beta-hydroxysteroid dehydrogenase 14
Weight
28.317kDa
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
17 betaHSD14; 17 beta-HSD14; dehydrogenase/reductase (SDR family) member 10; Dehydrogenase/reductase SDR family member 10; DHRS10; DHRS1017-beta-hydroxysteroid dehydrogenase 14; EC 1.1.1.-; HSD17B14; hydroxysteroid (17-beta) dehydrogenase 14,17-beta-hydroxysteroid dehydrogenase DHRS10; retinal short-chain dehydrogenase/reductase 3; Retinal short-chain dehydrogenase/reductase retSDR3; retSDR3; RETSDR3,17-beta-HSD 14; SDR3; SDR47C1; short chain dehydrogenase/reductase family 47C, member 1 HSD17B14 DHRS10, SDR47C1, retSDR3 hydroxysteroid 17-beta dehydrogenase 14 17-beta-hydroxysteroid dehydrogenase 14|17-beta-HSD 14|17-beta-hydroxysteroid dehydrogenase DHRS10|dehydrogenase/reductase (SDR family) member 10|retinal short-chain dehydrogenase/reductase 3|retinal short-chain dehydrogenase/reductase retSDR3|short chain dehydrogenase/reductase family 47C member 1|testicular tissue protein Li 52
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on HSD17B14, check out the HSD17B14 Infographic
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In this infographic, you will see the following information for HSD17B14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For HSD17B14 (NM_016246) Human Over-expression Lysate (LS051171)
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