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Product Info Summary
| SKU: | EK2134 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human ASAH2 ELISA Kit PicoKine®
View all ASAH2/N-acylsphingosine Amidohydrolase-2 ELISA kits
SKU/Catalog Number
EK2134
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ASAH2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ASAH2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ASAH2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2134)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: F99-I780
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2134 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2134 is reactive to Asah2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2134 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ASAH2/N-acylsphingosine Amidohydrolase-2
Neutral ceramidase is an enzyme that in humans is encoded by the ASAH2 gene. Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2134-CAP | Anti-Human Asah2 Pre-coated 96-well strip microplate | 1 |
| EK2134-ST | Human Asah2 Standard | 2 vials, 10 ng/tube |
| EK2134-DA | Human Asah2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.097 | 0.252 | 0.391 | 0.594 | 0.887 | 1.451 | 2.139 | 2.394 |
Data Example Images
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Human ASAH2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ASAH2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 171 | 645 | 1669 | 147 | 593 | 1596 |
| Standard deviation | 12.14 | 44.51 | 108.49 | 8.09 | 34.99 | 100.55 |
| CV(%) | 7.1% | 6.9% | 6.5% | 5.5% | 5.9% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ASAH2/N-acylsphingosine Amidohydrolase-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 171 | 154 | 166 | 159 | 163 | 5.36 | 3.3% |
| Sample 2 | 645 | 652 | 670 | 701 | 667 | 30.02 | 4.5% |
| Sample 3 | 1669 | 1466 | 1624 | 1563 | 1581 | 64.8 | 4.1% |
Protein Target Info & Infographic
Gene/Protein Information For Asah2 (Source: Uniprot.Org, NCBI)
Gene Name
Asah2
Full Name
Neutral ceramidase
Weight
83.509kDa
Superfamily
neutral ceramidase family
Alternative Names
ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; ASAH2; BCDase; HNAC1; LCDase; Nacylsphingosine Amidohydrolase2; NCDase; N-CDase Asah2|AI585898|N-acylsphingosine amidohydrolase 2|neutral ceramidase|N-CDase|NCDase|acylsphingosine deacylase 2|neutral/alkaline ceramidase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Asah2, check out the Asah2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Asah2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ASAH2 ELISA Kit PicoKine® (EK2134)
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