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Product Info Summary
| SKU: | EK2217 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human B7-H2/ICOSL ELISA Kit PicoKine®
View all B7-H2/ICOSLG ELISA kits
SKU/Catalog Number
EK2217
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human B7-H2/ICOSL PicoKine ELISA Kit® (96 Tests). Quantitate Human B7-H2/ICOSL in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 300 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human B7-H2/ICOSL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2217)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D19-S258
Sensitivity
<300 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2217 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2217 is reactive to ICOSLG in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2217 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of B7-H2/ICOSLG
ICOS ligand (ICOSLG, ICOSL or GL50) is a protein that in humans is encoded by the ICOSLG gene located at chromosome 21. Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2217-CAP | Anti-Human ICOSLG Pre-coated 96-well strip microplate | 1 |
| EK2217-ST | Human ICOSLG Standard | 2 vials, 40 ng/tube |
| EK2217-DA | Human ICOSLG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
| O.D. | 0.019 | 0.115 | 0.193 | 0.410 | 0.737 | 1.176 | 1.355 | 2.053 |
Data Example Images
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Human ICOSLG PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human B7-H2/ICOSL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 2015 | 4613 | 23467 | 1987 | 4563 | 21547 |
| Standard deviation | 90.68 | 281.39 | 1608 | 97.36 | 333.1 | 1335.91 |
| CV(%) | 4.5% | 6.1% | 6.8% | 4.9% | 7.3% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of B7-H2/ICOSLG in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 2015 | 2162 | 1984 | 1947 | 2027 | 99.32 | 4.9% |
| Sample 2 | 4613 | 4696 | 4536 | 4799 | 4661 | 270.34 | 5.8% |
| Sample 3 | 23647 | 25479 | 24519 | 22796 | 24020 | 1393.17 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For ICOSLG (Source: Uniprot.Org, NCBI)
Gene Name
ICOSLG
Full Name
ICOS ligand
Weight
33.349kDa
Superfamily
immunoglobulin superfamily
Alternative Names
B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand ICOSLG B7-H2, B7H2, B7RP-1, B7RP1, B7h, CD275, GL50, ICOS-L, ICOSL, LICOS inducible T cell costimulator ligand ICOS ligand|B7 homolog 2|B7 homologue 2|B7-like protein Gl50|B7-related protein 1|transmembrane protein B7-H2 ICOS ligand
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ICOSLG, check out the ICOSLG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ICOSLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human B7-H2/ICOSL ELISA Kit PicoKine® (EK2217)
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