Human CD21/CR2 PicoKine® Quick ELISA Kit

Product Name

Human CD21/CR2 PicoKine® Quick ELISA Kit

See all CR2 products

SKU/Catalog Number

FEK1315

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD21/CR2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CD21/CR2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1315)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Ile21-71

Sensitivity

<20 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1315 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1315 is reactive to CR2 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK1315 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CR2

Complement receptor type 2 (CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).

Kit Components

Catalog number	Description	Quantity	Volume
FEK1315-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK1315-ST	Human CR2 Standard	2	50 ng/tube
FEK1315-A	Human CR2 Antibody A	1	3ml
FEK1315-B	Human CR2 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.014	0.096	0.168	0.317	0.565	0.938	1.757	2.382

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CR2 in ELISA kits from four different production batches/lots.

Gene/Protein Information For CR2 (Source: Uniprot.Org, NCBI)

Alternative Names

C3DR; C3DRSLEB9; CD21 antigen; CD21; Complement C3d receptor; complement component (3d/Epstein Barr virus) receptor 2; complement receptor type 2; CR2; EBV receptor; Epstein-Barr virus receptor CR2 C3DR, CD21, CR, CVID7, SLEB9 complement C3d receptor 2 complement receptor type 2|EBV receptor|complement component (3d/Epstein Barr virus) receptor 2|complement component 3d receptor 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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