Human CFHR5 PicoKine® Quick ELISA Kit

Product Name

Human CFHR5 PicoKine® Quick ELISA Kit

See all Complement Factor H-related 5/CFHR5 products

SKU/Catalog Number

FEK1933

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CFHR5 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CFHR5 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 0.1 ng/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CFHR5 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1933)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-E569

Sensitivity

<0.1 ng/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1933 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1933 is reactive to CFHR5 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA) and urine

Application Guarantee

FEK1933 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Complement Factor H-related 5/CFHR5

Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).

Kit Components

Catalog number	Description	Quantity	Volume
FEK1933-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK1933-ST	Human Complement Factor H-related 5/CFHR5 Standard	2	50 ng/tube
FEK1933-A	Human CFHR5 Antibody A	1	3ml
FEK1933-B	Human CFHR5 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.028	0.269	0.470	0.722	1.123	1.623	1.985	2.387

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Complement Factor H-related 5/CFHR5 in ELISA kits from four different production batches/lots.

Gene/Protein Information For CFHR5 (Source: Uniprot.Org, NCBI)

Alternative Names

CFHL5; CFHL5factor H-related protein 5; CFHR5; Complement Factor Hrelated 5; Complement Factor H-related 5; FHR5; FHR-5complement factor H-related protein 5; FHR5MGC133240; FLJ10549 CFHR5 CFHL5D, FHR-5, FHR5, CFHR5 complement factor H related 5 complement factor H-related protein 5|factor H-related protein 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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