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Product Info Summary
| SKU: | EK2170 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CNTFR ELISA Kit PicoKine®
SKU/Catalog Number
EK2170
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CNTFR ELISA Kit PicoKine™ (96 Tests). Quantitate Human CNTFR in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CNTFR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2170)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q23-S342
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2170 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2170 is reactive to CNTFR in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2170 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cntfr
The ciliary neurotrophic factor receptor, also known as CNTFR, binds the ciliary neurotrophic factor. This gene encodes a member of the type 1 cytokine receptor family. The encoded protein is the ligand-specific component of a tripartite receptor for ciliary neurotrophic factor, which plays a critical role in neuronal cell survival, differentiation and gene expression. Binding of ciliary neurotrophic factor to the encoded protein recruits the transmembrane components of the receptor, gp130 and leukemia inhibitory factor receptor, facilitating signal transduction. Single nucleotide polymorphisms in this gene may be associated with variations in muscle strength, as well as early onset of eating disorders. Alternatively spliced transcript variants have been observed for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2170-CAP | Anti-Human CNTFR Pre-coated 96-well strip microplate | 1 |
| EK2170-ST | Human CNTFR Standard | 2 vials, 10 ng/tube |
| EK2170-DA | Human CNTFR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.059 | 0.082 | 0.109 | 0.188 | 0.411 | 0.675 | 1.378 | 2.079 |
Data Example Images
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Human CNTFR PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cntfr, Dilution ratio of 1:1, concentration in serum and plasma is 133-252 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CNTFR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 112 | 671 | 1458 | 110 | 709 | 1522 |
| Standard deviation | 4.82 | 50.33 | 59.78 | 7.37 | 65.23 | 65.15 |
| CV (%) | 4.3% | 7.5% | 4.1% | 6.7% | 9.2% | 4.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cntfr in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 112 | 120 | 118 | 109 | 115 | 4.13 | 3.6% |
| Sample 2 | 671 | 669 | 720 | 705 | 691 | 42.17 | 6.1% |
| Sample 3 | 1458 | 1527 | 1469 | 1566 | 1505 | 66.22 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For CNTFR (Source: Uniprot.Org, NCBI)
Gene Name
CNTFR
Full Name
Ciliary neurotrophic factor receptor subunit alpha
Weight
40.633kDa
Superfamily
type I cytokine receptor family
Alternative Names
CNTF R alpha; CNTFR alpha; CNTFR; CNTFRa CNTFR ciliary neurotrophic factor receptor ciliary neurotrophic factor receptor subunit alpha|CNTF receptor subunit alpha|CNTFR-alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CNTFR, check out the CNTFR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CNTFR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CNTFR ELISA Kit PicoKine® (EK2170)
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