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Product Info Summary
| SKU: | EK2179 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human COLEC12 ELISA Kit PicoKine®
View all CL-P1/COLEC12 ELISA kits
SKU/Catalog Number
EK2179
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human COLEC12 ELISA Kit PicoKine™ (96 Tests). Quantitate Human COLEC12 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human COLEC12 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2179)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L57-L742
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2179 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2179 is reactive to COLEC12 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2179 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CL-P1/COLEC12
Collectin-12, also known as collectin subfamily member 12, is a collectin protein that in humans is encoded by the COLEC12 gene.This gene encodes a member of the C-lectin family, proteins that possess collagen-like sequences and carbohydrate recognition domains. This protein is a scavenger receptor that displays several functions associated with host defense. It can bind to carbohydrate antigens on microorganisms, facilitating their recognition and removal. It also mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein by vascular endothelial cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2179-CAP | Anti-Human COLEC12 Pre-coated 96-well strip microplate | 1 |
| EK2179-ST | Human COLEC12 Standard | 2 vials, 10 ng/tube |
| EK2179-DA | Human COLEC12 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.037 | 0.078 | 0.181 | 0.347 | 0.823 | 1.368 | 1.875 | 2.362 |
Data Example Images
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Human COLEC12 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human COLEC12 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 352 | 1277 | 4565 | 385 | 1365 | 4632 |
| Standard deviation | 19.36 | 71.51 | 146.08 | 23.49 | 94.19 | 213.07 |
| CV(%) | 5.5% | 5.6% | 3.2% | 6.1% | 6.9% | 4.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CL-P1/COLEC12 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 352 | 365 | 363 | 371 | 363 | 19.95 | 5.5% |
| Sample 2 | 1277 | 1265 | 1302 | 1287 | 1283 | 75.68 | 5.9% |
| Sample 3 | 4565 | 4542 | 4615 | 4787 | 4627 | 286.89 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For COLEC12 (Source: Uniprot.Org, NCBI)
Gene Name
COLEC12
Full Name
Collectin-12
Weight
81.515kDa
Alternative Names
CL-P1; CLP1hCL-P1; COLEC12; collectin placenta 1; Collectin placenta protein 1; collectin sub-family member 12; collectin-12; NSR2; Nurse cell scavenger receptor 2; SCARA4; SCARA4NSR2; Scavenger receptor class A member 4; scavenger receptor class A, member 4; SRCL Type I; SRCLScavenger receptor with C-type lectin COLEC12 CLP1, NSR2, SCARA4, SRCL collectin subfamily member 12 collectin-12|collectin placenta protein 1|collectin sub-family member 12|hCL-P1|nurse cell scavenger receptor 2|scavenger receptor class A, member 4|scavenger receptor with C-type lectin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on COLEC12, check out the COLEC12 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for COLEC12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human COLEC12 ELISA Kit PicoKine® (EK2179)
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