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Product Info Summary
| SKU: | FEK0913 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human COMP/Thrombospondin 5 PicoKine® Quick ELISA Kit
See all COMP/Thrombospondin-5 products
SKU/Catalog Number
FEK0913
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human COMP/Thrombospondin 5 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human COMP/Thrombospondin 5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human COMP/Thrombospondin 5 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0913)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q21-A757
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0913 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0913 is reactive to COMP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK0913 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of COMP/Thrombospondin-5
Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0913-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0913-ST | Human COMP/Thrombospondin-5 Standard | 2 | 10 ng/tube |
| FEK0913-A | Human COMP Antibody A | 1 | 3ml |
| FEK0913-B | Human COMP Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.051 | 0.104 | 0.136 | 0.209 | 0.352 | 0.611 | 1.059 | 1.804 |
Data Example Images
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Human COMP PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 257 | 1962 | 4987 | 269 | 1888 | 4741 |
| Standard deviation | 13.62 | 107.91 | 204.47 | 15.06 | 109.5 | 279.72 |
| CV(%) | 5.3% | 5.5% | 4.1% | 5.6% | 5.8% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of COMP/Thrombospondin-5 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 257 | 303 | 298 | 273 | 283 | 11.03 | 3.9% |
| Sample 2 | 1962 | 2068 | 2165 | 2214 | 2102 | 58.86 | 2.8% |
| Sample 3 | 4987 | 4856 | 4713 | 4651 | 4802 | 259.29 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For COMP (Source: Uniprot.Org, NCBI)
Gene Name
COMP
Full Name
Cartilage oligomeric matrix protein
Weight
82.86kDa
Superfamily
thrombospondin family
Alternative Names
cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5 COMP CTS2, EDM1, EPD1, MED, PSACH, THBS5, TSP5 cartilage oligomeric matrix protein cartilage oligomeric matrix protein|cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple)|pseudoachondroplasia (epiphyseal dysplasia 1, multiple)|thrombospondin-5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on COMP, check out the COMP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for COMP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human COMP/Thrombospondin 5 PicoKine® Quick ELISA Kit (FEK0913)
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