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Product Info Summary
| SKU: | EK2143 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma(heparin, EDTA, citrate) and urine |
Product info
Product Name
Human Complement C2 ELISA Kit PicoKine®
SKU/Catalog Number
EK2143
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Complement C2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human C2 in cell culture supernatants, serum, plasma(heparin, EDTA, citrate) and urine. Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Complement C2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2143)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A21-L752
Sensitivity
<20 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2143 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2143 is reactive to C2 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma(heparin, EDTA, citrate) and urine
Application Guarantee
EK2143 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of C2
Complement C2 is a protein that in humans is encoded by the C2 gene. Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2143-CAP | Anti-Human C2 Pre-coated 96-well strip microplate | 1 |
| EK2143-ST | Human C2 Standard | 2 vials, 20 ng/tube |
| EK2143-DA | Human C2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.011 | 0.039 | 0.087 | 0.186 | 0.325 | 0.688 | 1.052 | 2.036 |
Data Example Images
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Human Complement C2 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Complement C2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 785 | 1654 | 10235 | 766 | 1641 | 10634 |
| Standard deviation | 35.33 | 71.12 | 521.99 | 24.51 | 59.08 | 521.07 |
| CV(%) | 4.5% | 4.3% | 5.1% | 3.2% | 3.6% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of C2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 785 | 774 | 769 | 782 | 778 | 22.55 | 2.9% |
| Sample 2 | 1654 | 1723 | 1645 | 1697 | 1680 | 43.67 | 2.6% |
| Sample 3 | 10235 | 10156 | 10619 | 10665 | 10306 | 371.03 | 3.6% |
Protein Target Info & Infographic
Gene/Protein Information For C2 (Source: Uniprot.Org, NCBI)
Gene Name
C2
Full Name
Complement C2
Weight
83.268kDa
Superfamily
peptidase S1 family
Alternative Names
Complement Component C2b C2 ARMD14, CO2 complement C2 complement C2|C3/C5 convertase|complement component 2|complement component C2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on C2, check out the C2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for C2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Complement C2 ELISA Kit PicoKine® (EK2143)
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