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Product Info Summary
| SKU: | EK2190 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human DSG3 ELISA Kit PicoKine®
View all Desmoglein-3 ELISA kits
SKU/Catalog Number
EK2190
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human DSG3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human DSG3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human DSG3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2190)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E50-R615
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2190 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2190 is reactive to DSG3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2190 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Desmoglein-3
Desmoglein-3 is a protein that in humans is encoded by the DSG3 gene. This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2190-CAP | Anti-Human DSG3 Pre-coated 96-well strip microplate | 1 |
| EK2190-ST | Human DSG3 Standard | 2 vials, 10 ng/tube |
| EK2190-DA | Human DSG3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.022 | 0.059 | 0.152 | 0.261 | 0.488 | 0.892 | 1.265 | 2.052 |
Data Example Images
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Human DSG3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human DSG3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 124 | 268 | 652 | 115 | 279 | 663 |
| Standard deviation | 6.32 | 9.65 | 25.43 | 4.83 | 13.67 | 38.45 |
| CV(%) | 5.1% | 3.6% | 3.9% | 4.2% | 4.9% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Desmoglein-3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 124 | 118 | 109 | 112 | 116 | 3.82 | 3.3% |
| Sample 2 | 268 | 272 | 259 | 266 | 266 | 12.51 | 4.7% |
| Sample 3 | 652 | 642 | 638 | 674 | 652 | 36.48 | 5.6% |
Protein Target Info & Infographic
Gene/Protein Information For DSG3 (Source: Uniprot.Org, NCBI)
Gene Name
DSG3
Full Name
Desmoglein-3
Weight
107.533kDa
Alternative Names
Cadherin family member 6; CDHF6; CDHF6130-kD pemphigus vulgaris antigen; desmoglein 3 (pemphigus vulgaris antigen); desmoglein 3; Desmoglein3; Desmoglein-3; DKFZp686P23184,130 kDa pemphigus vulgaris antigen; DSG3; pemphigus vulgaris antigen; PVA DSG3 ABOLM, CDHF6, PVA desmoglein 3 desmoglein-3|130 kDa pemphigus vulgaris antigen|cadherin family member 6
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DSG3, check out the DSG3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DSG3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human DSG3 ELISA Kit PicoKine® (EK2190)
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