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Product Info Summary
| SKU: | EK2198 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human F10 ELISA Kit PicoKine®
View all Coagulation Factor X ELISA kits
SKU/Catalog Number
EK2198
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human F10 ELISA Kit PicoKine™ (96 Tests). Quantitate Human F10 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human F10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2198)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L24-K488
Sensitivity
<20 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2198 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2198 is reactive to F10 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2198 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Coagulation Factor X
This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2198-CAP | Anti-Human F10 Pre-coated 96-well strip microplate | 1 |
| EK2198-ST | Human F10 Standard | 2 vials, 50 ng/tube |
| EK2198-DA | Human F10 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.012 | 0.091 | 0.188 | 0.352 | 0.755 | 1.374 | 1.634 | 2.041 |
Data Example Images
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Human F10 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human F10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1963 | 3865 | 21664 | 2014 | 413 | 21874 |
| Standard deviation | 106 | 224.17 | 866.56 | 126.88 | 26.02 | 1203.07 |
| CV(%) | 5.4% | 5.8% | 4% | 6.3% | 6.3% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Coagulation Factor X in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1462 | 1658 | 1531 | 1596 | 1562 | 98.39 | 6.3% |
| Sample 2 | 8562 | 8960 | 8216 | 8472 | 8553 | 496.05 | 5.8% |
| Sample 3 | 20623 | 22361 | 21634 | 20478 | 21274 | 1255.17 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For F10 (Source: Uniprot.Org, NCBI)
Gene Name
F10
Full Name
Coagulation factor X
Weight
54.732kDa
Superfamily
peptidase S1 family
Alternative Names
Cf10; Coagulation Factor X; EC 3.4.21; EC 3.4.21.6; F10; factor Xa; FX; FXa; Prothrombinase; Stuart Factor; Stuart-Prower factor F10 FX, FXA coagulation factor X coagulation factor X|Stuart-Prower factor|factor X|factor Xa|prothrombinase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on F10, check out the F10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human F10 ELISA Kit PicoKine® (EK2198)
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