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Product Info Summary
| SKU: | A00641 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody
SKU/Catalog Number
A00641
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody catalog # A00641. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00641)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FA10. AA range:216-265
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00641 is reactive to F10 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
54.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00641 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of rat cells using Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody
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Western blot analysis of lysates from rat liver cells, using FA10 (activated heavy chain, Cleaved-Ile235) Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody (A00641)
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5 Customer Q&As for Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody
Question
We are currently using anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The anti-Cleaved-Factor Xa activated HC (I235) antibody (A00641) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-05
Question
Is a blocking peptide available for product anti-Cleaved-Factor Xa activated HC (I235) antibody (A00641)?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
We do provide the blocking peptide for product anti-Cleaved-Factor Xa activated HC (I235) antibody (A00641). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-07
Question
I have a question about product A00641, anti-Cleaved-Factor Xa activated HC (I235) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00641 anti-Cleaved-Factor Xa activated HC (I235) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-20
Question
Will anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 work for WB with liver?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
According to the expression profile of liver, F10 is highly expressed in liver. So, it is likely that anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 will work for WB with liver.
Boster Scientific Support
Answered: 2018-12-17
Question
We want to test anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 on rat liver for research purposes, then I may be interested in using anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Li
Verified customer
Asked: 2013-02-15
Answer
The products we sell, including anti-Cleaved-Factor Xa activated HC (I235) antibody A00641, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-02-15
