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Product Info Summary
| SKU: | EK2199 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human F11 ELISA Kit PicoKine®
View all Coagulation Factor XI ELISA kits
SKU/Catalog Number
EK2199
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human F11 ELISA Kit PicoKine™ (96 Tests). Quantitate Human F11 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human F11 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2199)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E19-V625
Sensitivity
<20 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2199 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2199 is reactive to F11 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2199 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Coagulation Factor XI
Factor XI or plasma thromboplastin antecedent is the zymogen form of factor XIa, one of the enzymes of the coagulation cascade. Like many other coagulation factors, it is a serine protease. In humans, Factor XI is encoded by the F11 gene. This gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2199-CAP | Anti-Human F11 Pre-coated 96-well strip microplate | 1 |
| EK2199-ST | Human F11 Standard | 2 vials, 50 ng/tube |
| EK2199-DA | Human F11 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.006 | 0.058 | 0.125 | 0.347 | 0.659 | 1.244 | 1.523 | 1.922 |
Data Example Images
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Human F11 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human F11 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1652 | 3264 | 6973 | 1687 | 3645 | 7524 |
| Standard deviation | 7269 | 159.94 | 362.6 | 92.79 | 167.67 | 428.87 |
| CV(%) | 4.4% | 4.9% | 5.2% | 5.5% | 4.6% | 5.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Coagulation Factor XI in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1652 | 1773 | 1679 | 1834 | 1735 | 76.32 | 4.4% |
| Sample 2 | 3264 | 3645 | 3579 | 3421 | 3477 | 166.91 | 4.8% |
| Sample 3 | 6973 | 6872 | 6721 | 7094 | 6915 | 359.58 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For F11 (Source: Uniprot.Org, NCBI)
Gene Name
F11
Full Name
Coagulation factor XI
Weight
70.109kDa
Superfamily
peptidase S1 family
Alternative Names
Coagulation Factor XI; EC 3.4.21; EC 3.4.21.27; FXIPlasma thromboplastin antecedent; MGC141891; PTA F11 FXI, PTA coagulation factor XI coagulation factor XI|coagualtion factor XI|plasma thromboplastin antecedent
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on F11, check out the F11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human F11 ELISA Kit PicoKine® (EK2199)
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