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Product Info Summary
| SKU: | EK1930 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human FCRL1 ELISA Kit PicoKine®
View all FCRL1/FcRH1 ELISA kits
SKU/Catalog Number
EK1930
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FCRL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCRL1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FCRL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1930)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A17-S307
Sensitivity
<20 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1930 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1930 is reactive to FCRL1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1930 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCRL1/FcRH1
Fc receptor-like protein 1 is a protein that in humans is encoded by the FCRL1 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1930-CAP | Anti-Human FCRL1 Pre-coated 96-well strip microplate | 1 |
| EK1930-ST | Human FCRL1 Standard | 2 vials, 10 ng/tube |
| EK1930-DA | Human FCRL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.017 | 0.074 | 0.127 | 0.226 | 0.480 | 0.822 | 1.297 | 2.024 |
Data Example Images
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Human FCRL1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FCRL1/FcRH1, Dilution ratio of 1:1, concentration in serum and plasma is 180-1000 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FCRL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 42 | 182 | 837 | 51 | 193 | 947 |
| Standard deviation | 2.18 | 9.65 | 46.04 | 3.11 | 12.35 | 63.45 |
| CV (%) | 5.2% | 5.3% | 5.5% | 6.1% | 6.4% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FCRL1/FcRH1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 42 | 40 | 42 | 45 | 42 | 1.78 | 4.2% |
| Sample 2 | 182 | 167 | 169 | 161 | 169 | 7.66 | 4.5% |
| Sample 3 | 837 | 797 | 842 | 900 | 844 | 36.73 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For FCRL1 (Source: Uniprot.Org, NCBI)
Gene Name
FCRL1
Full Name
Fc receptor-like protein 1
Weight
46.936kDa
Alternative Names
BXMH1; CD307a antigen; CD307a; Fc receptor homolog 1; Fc receptor-like 1; FcRH1; FCRH1DKFZp667O1421; FCRL1; FcR-like protein 1; hIFGP1; IFGP family protein 1; IFGP1; IFGP1Fc receptor-like protein 1; Immune receptor translocation-associated protein 5; IRTA5; IRTA5immunoglobulin superfamily Fc receptor, gp42 FCRL1 CD307a, FCRH1, IFGP1, IRTA5 Fc receptor like 1 Fc receptor-like protein 1|IFGP family protein 1|fc receptor homolog 1|fcR-like protein 1|hIFGP1|immune receptor translocation-associated protein 5|immunoglobulin superfamily Fc receptor, gp42
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FCRL1, check out the FCRL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCRL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FCRL1 ELISA Kit PicoKine® (EK1930)
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No publications found for EK1930
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human FCRL1 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human FCRL1 Picokine® ELISA Kit (Catalog # EK1930)?
W. Clark
Verified customer
Asked: 2019-09-02
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of FCRL1 causing degradation of its protein structure. FCRL1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FCRL1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-09-02
Question
Q: if the enzyme conjugated FCRL1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FCRL1 antigen?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-07-23
Question
Q: What is the optimal O.D. value for FCRL1 ELISA kit? I used your FCRL1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain FCRL1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FCRL1 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of FCRL1 that can be declared/interpreted as positive by the above standard is 20pg/ml.
Boster Scientific Support
Answered: 2018-03-09
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
C. Kaur
Verified customer
Asked: 2017-08-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-08-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-05-31
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring FCRL1 using the EK1930 Human FCRL1 Picokine® ELISA Kit?
C. Gonzalez
Verified customer
Asked: 2014-09-18
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2014-09-18
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
R. Mitchell
Verified customer
Asked: 2014-06-30
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-06-30
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of FCRL1 in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
C. Sunder
Verified customer
Asked: 2014-06-21
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FCRL1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-06-21
