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Product Info Summary
| SKU: | EK1976 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human FCRL2 ELISA Kit PicoKine®
View all FCRL2/FcRH2 ELISA kits
SKU/Catalog Number
EK1976
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FCRL2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCRL2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FCRL2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1976)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0, Immunogen sequence: L20-V401
Sensitivity
<50 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1976 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1976 is reactive to FCRL2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1976 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCRL2/FcRH2
Fc receptor-like protein 2 is a protein that in humans is encoded by the FCRL2 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains one immunoreceptor-tyrosine activation motif and two immunoreceptor-tyrosine inhibitory motifs. This protein may be a prognostic marker for chronic lymphocytic leukemia. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1976-CAP | Anti-Human FCRL2 Pre-coated 96-well strip microplate | 1 |
| EK1976-ST | Human FCRL2 Standard | 2 vials, 10 ng/tube |
| EK1976-DA | Human FCRL2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.009 | 0.026 | 0.067 | 0.167 | 0.361 | 0.870 | 1.803 | 2.639 |
Data Example Images
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Human FCRL2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FCRL2/FcRH2, Dilution ratio of 1:1, concentration in serum and plasma is 250-550 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FCRL2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 44 | 136 | 578 | 53 | 168 | 605 |
| Standard deviation | 3.17 | 8.57 | 37.17 | 2.39 | 7.06 | 32.07 |
| CV (%) | 7.2% | 6.3% | 6.4% | 4.5% | 4.2% | 5.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FCRL2/FcRH2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 44 | 50 | 48 | 59 | 50 | 2.56 | 5.1% |
| Sample 2 | 136 | 152 | 144 | 146 | 145 | 4.77 | 3.3% |
| Sample 3 | 578 | 602 | 594 | 585 | 590 | 45.59 | 7.9% |
Protein Target Info & Infographic
Gene/Protein Information For FCRL2 (Source: Uniprot.Org, NCBI)
Gene Name
FCRL2
Full Name
Fc receptor-like protein 2
Weight
55.542kDa
Alternative Names
CD307b antigen; CD307b; Fc receptor homolog 2; Fc receptor-like 2; FcRH2; FCRH2SPAP1B; FCRL2; FcR-like protein 2; IFGP family protein 4; IFGP4; Immunoglobulin receptor translocation-associated protein 4; immunoglobulin superfamily Fc receptor, gp42; IRTA4; IRTA4SPAP1C; SH2 domain containing phosphatase anchor protein 1; SH2 domain-containing phosphatase anchor protein 1Fc receptor-like protein 2; SPAP1; SPAP1A; SPAP1immune receptor translocation-associated protein 4 FCRL2 CD307b, FCRH2, IFGP4, IRTA4, SPAP1, SPAP1A, SPAP1B, SPAP1C Fc receptor like 2 Fc receptor-like protein 2|IFGP family protein 4|SH2 domain containing phosphatase anchor protein 1|fc receptor homolog 2|immune receptor translocation-associated protein 4|immunoglobulin receptor translocation-associated protein 4|immunoglobulin superfamily Fc receptor, gp42
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FCRL2, check out the FCRL2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCRL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FCRL2 ELISA Kit PicoKine® (EK1976)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human FCRL2 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for FCRL2 ELISA kit? I performed your FCRL2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain FCRL2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-08-23
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FCRL2 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of FCRL2 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-08-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Dayal
Verified customer
Asked: 2020-01-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-01-05
Question
Q: can you recommend the dilution ratio of serum samples for detection of FCRL2 in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-01-05
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FCRL2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-01-05
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-06-04
Question
Q: which procedure should I follow in order to thaw whole blood sample for FCRL2 ELISA after freezing?
K. Williams
Verified customer
Asked: 2018-10-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FCRL2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-10-20
Question
Q: how do I analyze ELISA data? I measured FCRL2 level in serum.
L. Jackson
Verified customer
Asked: 2018-08-06
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-06
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
H. Harris
Verified customer
Asked: 2018-02-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-02-10
Question
Q: is it okay to use citrate plasma as samples in Human FCRL2 Picokine® ELISA Kit (Catalog # EK1976)?
A. Tiwari
Verified customer
Asked: 2016-05-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of FCRL2 causing disruption of its protein structure. FCRL2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the FCRL2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-05-22
