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Product Info Summary
| SKU: | EK2234 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human Galectin-8 ELISA Kit PicoKine®
View all Galectin-8 ELISA kits
SKU/Catalog Number
EK2234
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Galectin-8 PicoKine ELISA Kit® (96 Tests). Quantitate Human Galectin-8 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 25 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Galectin-8 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2234)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-W317
Sensitivity
<25 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2234 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2234 is reactive to LGALS8 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2234 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Galectin-8
Galectin-8 is a protein of the galectin family that in humans is encoded by the LGALS8 gene. This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2234-CAP | Anti-Human LGALS8 Pre-coated 96-well strip microplate | 1 |
| EK2234-ST | Human LGALS8 Standard | 2 vials, 10 ng/tube |
| EK2234-DA | Human LGALS8 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1,500 | 3,000 |
| O.D. | 0.003 | 0.066 | 0.106 | 0.212 | 0.469 | 0.896 | 1.220 | 1.890 |
Data Example Images
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Human Galectin-8 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Galectin-8 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 48 | 146 | 945 | 89 | 144 | 955 |
| Standard deviation | 2.07 | 6.58 | 25.04 | 2.6 | 10.87 | 28.75 |
| CV(%) | 4.3% | 4.5% | 2.7% | 2.9% | 7.6% | 3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Galectin-8 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 48 | 79 | 67 | 85 | 70 | 4.3 | 6.2% |
| Sample 2 | 146 | 177 | 153 | 123 | 150 | 7.73 | 5.2% |
| Sample 3 | 945 | 1007 | 955 | 1262 | 1042 | 73.69 | 7.1% |
Protein Target Info & Infographic
Gene/Protein Information For LGALS8 (Source: Uniprot.Org, NCBI)
Gene Name
LGALS8
Full Name
Galectin-8
Weight
35.808kDa
Alternative Names
GAL8; Gal-8; galectin 8; Galectin8; Galectin-8; galectin-8g; lectin, galactoside-binding, soluble, 8; LGALS8; PCTA1; PCTA-1Po66 carbohydrate-binding protein; Po66 carbohydrate binding protein; Po66-CBP; Prostate carcinoma tumor antigen 1 LGALS8 Gal-8, PCTA-1, PCTA1, Po66-CBP galectin 8 galectin-8|Po66 carbohydrate binding protein|galectin-8g|lectin, galactoside-binding, soluble, 8|po66 carbohydrate-binding protein|prostate carcinoma tumor antigen 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LGALS8, check out the LGALS8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LGALS8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Galectin-8 ELISA Kit PicoKine® (EK2234)
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