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Product Info Summary
| SKU: | EK2212 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human GPVI ELISA Kit PicoKine®
SKU/Catalog Number
EK2212
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GPVI Kit PicoKine™ (96 Tests). Quantitate Human GP6 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GPVI ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2212)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q21-K267
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2212 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2212 is reactive to GP6 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2212 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GPVI
Glycoprotein VI (platelet), also known as GPVI, is a glycoprotein receptor for collagen which is expressed in platelets. In humans, glycoprotein VI is encoded by the GPVI gene. This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2212-CAP | Anti-Human GP6 Pre-coated 96-well strip microplate | 1 |
| EK2212-ST | Human GP6 Standard | 2 vials, 10 ng/tube |
| EK2212-DA | Human GP6 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.027 | 0.078 | 0.141 | 0.267 | 0.423 | 0.968 | 1.375 | 2.062 |
Data Example Images
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Human GPVI PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GPVI ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 389 | 1264 | 4123 | 335 | 1396 | 4226 |
| Standard deviation | 17.51 | 70.78 | 131.94 | 18.43 | 80.97 | 207.07 |
| CV(%) | 4.5% | 5.6% | 3.2% | 5.5% | 5.8% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GPVI in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 389 | 377 | 365 | 358 | 372 | 21.96 | 5.9% |
| Sample 2 | 1264 | 1275 | 1289 | 1296 | 1281 | 65.33 | 5.1% |
| Sample 3 | 4123 | 4566 | 4693 | 4756 | 4535 | 276.6 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For GP6 (Source: Uniprot.Org, NCBI)
Gene Name
GP6
Full Name
Platelet glycoprotein VI
Weight
36.866kDa
Alternative Names
Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVI; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI GP6 BDPLT11, GPIV, GPVI glycoprotein VI platelet platelet glycoprotein VI|glycoprotein 6|platelet collagen receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GP6, check out the GP6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GP6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human GPVI ELISA Kit PicoKine® (EK2212)
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