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Product Info Summary
| SKU: | EK2245 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human IL22BP ELISA Kit PicoKine®
SKU/Catalog Number
EK2245
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL22BP PicoKine ELISA Kit® (96 Tests). Quantitate Human IL22BP in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 25 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL22BP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2245)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: T22-P231
Sensitivity
<25 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2245 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2245 is reactive to IL22RA2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2245 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL22RA2
This gene encodes a member of the class II cytokine receptor family. The encoded soluble protein specifically binds to and inhibits interleukin 22 activity by blocking the interaction of interleukin 22 with its cell surface receptor. The encoded protein may be important in the regulation of inflammatory response, and has been implicated in the regulation of tumorigenesis in the colon. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2245-CAP | Anti-Human IL22RA2 Pre-coated 96-well strip microplate | 1 |
| EK2245-ST | Human IL22RA2 Standard | 2 vials, 10 ng/tube |
| EK2245-DA | Human IL22RA2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1,500 | 3,000 |
| O.D. | 0.013 | 0.036 | 0.106 | 0.202 | 0.429 | 0.806 | 1.320 | 1.800 |
Data Example Images
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Human IL22BP PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL22BP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 54 | 151 | 1130 | 58 | 130 | 1247 |
| Standard deviation | 2.11 | 6.67 | 44.41 | 1.35 | 5.34 | 39.65 |
| CV(%) | 3.9% | 4.4% | 3.9% | 2.3% | 4.1% | 3.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL22RA2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 54 | 65 | 64 | 81 | 66 | 1.6 | 2.4% |
| Sample 2 | 151 | 158 | 136 | 134 | 145 | 2.92 | 2% |
| Sample 3 | 1130 | 1499 | 964 | 795 | 1097 | 30.06 | 2.7% |
Protein Target Info & Infographic
Gene/Protein Information For IL22RA2 (Source: Uniprot.Org, NCBI)
Gene Name
IL22RA2
Full Name
Interleukin-22 receptor subunit alpha-2
Weight
30.55kDa
Superfamily
type II cytokine receptor family
Alternative Names
class II cytokine receptor; CRF2-10; CRF2-S1IL22BP; CRF2-X; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; Cytokine receptor family type 2, soluble 1; IL-22 RA2; IL-22 receptor subunit alpha-2; IL22BP; IL-22BP; IL-22BPCRF2X; IL22RA2; IL-22RA2; IL-22R-alpha-2; interleukin 22 receptor, alpha 2; interleukin 22-binding protein; interleukin-22 receptor subunit alpha-2; Interleukin-22-binding protein; MGC150509; MGC150510; zcytoR16 IL22RA2 CRF2-10, CRF2-S1, CRF2X, IL-22BP, IL-22R-alpha-2, IL-22RA2, ZCYTOR16 interleukin 22 receptor subunit alpha 2 interleukin-22 receptor subunit alpha-2|cytokine receptor class-II member 10|cytokine receptor family type 2, soluble 1|interleukin 22 receptor, alpha 2|interleukin 22-binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL22RA2, check out the IL22RA2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL22RA2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL22BP ELISA Kit PicoKine® (EK2245)
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