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Product Info Summary
| SKU: | EK0817 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , human milk and saliva. |
Product info
Product Name
Human KLK5/Kallikrein-5 ELISA Kit PicoKine®
View all Kallikrein 5 ELISA kits
SKU/Catalog Number
EK0817
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human KLK5/Kallikrein 5 ELISA Kit PicoKine™ (96 Tests). Quantitate Human KLK5 in cell culture supernatants, serum, plasma (heparin, EDTA) , human milk and saliva. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human KLK5/Kallikrein-5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0817)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: V23-S293
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0817 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0817 is reactive to KLK5 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , human milk and saliva.
Application Guarantee
EK0817 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Kallikrein 5
Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0817-CAP | Anti-Human KLK5 Pre-coated 96-well strip microplate | 1 |
| EK0817-ST | Human KLK5 Standard | 2 vials, 10 ng/tube |
| EK0817-DA | Human KLK5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.042 | 0.165 | 0.259 | 0.357 | 0.803 | 1.081 | 1.605 | 1.988 |
Data Example Images
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Human KLK5 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Kallikrein 5, Dilution ratio of 1:1, concentration in serum and plasma is around 140 pg/ml..
Some articles we found to cite concentrations of Kallikrein 5 in samples: 12873991 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human KLK5/Kallikrein-5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 134 | 809 | 2161 | 146 | 747 | 2368 |
| Standard deviation | 5.36 | 33.97 | 133.98 | 8.32 | 32.12 | 149.18 |
| CV (%) | 4% | 4.2% | 6.2% | 5.7% | 4.3% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Kallikrein 5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 134 | 128 | 140 | 148 | 137 | 7.39 | 5.3% |
| Sample 2 | 809 | 798 | 776 | 808 | 797 | 13.27 | 1.6% |
| Sample 3 | 2161 | 2628 | 2234 | 2197 | 2305 | 188.26 | 8.1% |
Protein Target Info & Infographic
Gene/Protein Information For KLK5 (Source: Uniprot.Org, NCBI)
Gene Name
KLK5
Full Name
Kallikrein-5
Weight
32.02kDa
Superfamily
peptidase S1 family
Alternative Names
EC 3.4.21; EC 3.4.21.4; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme KLK5 KLK-L2, KLKL2, SCTE kallikrein related peptidase 5 kallikrein-5|kallikrein-like protein 2|stratum corneum tryptic enzyme
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on KLK5, check out the KLK5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KLK5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human KLK5/Kallikrein-5 ELISA Kit PicoKine® (EK0817)
Hello CJ!
No publications found for EK0817
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
6 Customer Q&As for Human KLK5/Kallikrein-5 ELISA Kit PicoKine®
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Kallikrein 5 in Human saliva? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
W. White
Verified customer
Asked: 2020-02-27
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Kallikrein 5 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-02-27
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-01-14
Question
Q: is it okay to use citrate plasma as samples in Human Kallikrein 5 Picokine® ELISA Kit (Catalog # EK0817)?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Kallikrein 5 causing degradation of its protein structure. Kallikrein 5 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Kallikrein 5 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-06-24
Question
Q: Can Kallikrein 5 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
C. Wang
Verified customer
Asked: 2019-06-06
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-06-06
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Kallikrein 5 level in saliva.
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-22
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-11-16
