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Product Info Summary
| SKU: | FEK0817 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human Kallikrein 5/KLK5 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0817
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Kallikrein 5/KLK5 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human KLK5 in cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva. Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Kallikrein 5/KLK5 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0817)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: V23-S293
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0817 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0817 is reactive to KLK5 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva
Application Guarantee
FEK0817 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Kallikrein 5
Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0817-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0817-ST | Human Kallikrein 5 Standard | 2 | 10 ng/tube |
| FEK0817-A | Human KLK5 Antibody A | 1 | 3ml |
| FEK0817-B | Human KLK5 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 |
| O.D. | 0.033 | 0.096 | 0.150 | 0.312 | 0.430 | 0.798 | 1.315 | 1.913 |
Data Example Images
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Human KLK5 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 139 | 814 | 2166 | 151 | 752 | 2373 |
| Standard deviation | 5.69 | 35.81 | 121.29 | 9.96 | 38.35 | 151.87 |
| CV(%) | 4.1% | 4.4% | 5.6% | 6.6% | 5.1% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Kallikrein 5 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 139 | 133 | 145 | 153 | 142 | 7.38 | 5.2% |
| Sample 2 | 814 | 803 | 781 | 813 | 802 | 13.63 | 1.7% |
| Sample 3 | 2166 | 2633 | 2239 | 2202 | 2310 | 189.42 | 8.2% |
Protein Target Info & Infographic
Gene/Protein Information For KLK5 (Source: Uniprot.Org, NCBI)
Gene Name
KLK5
Full Name
Kallikrein-5
Weight
32.02kDa
Superfamily
peptidase S1 family
Alternative Names
EC 3.4.21; EC 3.4.21.4; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme KLK5 KLK-L2, KLKL2, SCTE kallikrein related peptidase 5 kallikrein-5|kallikrein-like protein 2|stratum corneum tryptic enzyme
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on KLK5, check out the KLK5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KLK5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Kallikrein 5/KLK5 PicoKine® Quick ELISA Kit (FEK0817)
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