Human KLK5/Kallikrein 5 PicoKine™ ELISA Kit
|Size||96wells/kit, with removable strips.|
|Sample Type||cell culture supernates, serum, plasma(heparin , EDTA), human milk and saliva.|
|Product Name||Human KLK5/Kallikrein 5 PicoKine™ ELISA Kit|
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Size||96wells/kit, with removable strips.|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK5. 96wells/kit, with removable strips.|
|Cite This Product||Human KLK5/Kallikrein 5 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0817)|
|Sample Type||cell culture supernates, serum, plasma(heparin , EDTA), human milk and saliva..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: V23-S293|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|EK0817-CAP||96-well plate precoated with anti-Human KLK5 antibody||1|
|EK0817-ST||lyophilized recombinant Human KLK5 standard||10ng/tube|
|EK0817-DA||biotinylated anti-Human KLK5 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Human KLK5/Kallikrein 5 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Protein Target Info (Source: Uniprot.org)
|Tissue Specificity||Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin. .|
|Alternative Names||Kallikrein-5;3.4.21.-;Kallikrein-like protein 2;KLK-L2;Stratum corneum tryptic enzyme;KLK5;SCTE;UNQ570/PRO1132;|
|Molecular Weight||32019 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||May be involved in desquamation.|
|Background||Kallikrein gene 5(KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.|
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The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
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