Human KLK5/Kallikrein 5 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK5. 96wells/kit, with removable strips.
Human KLK5/Kallikrein 5 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(heparin , EDTA), human milk and saliva.|
|Sample Volume:||100μl per well|
|Product Name||Human KLK5/Kallikrein 5 ELISA Kit PicoKine™
See all Kallikrein 5 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Klk L2/KLK5 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin , EDTA), human milk and saliva. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Klk L2/KLK5 tissue specificity: Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin. .|
|Cite This Product||Human KLK5/Kallikrein 5 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0817)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin , EDTA), human milk and saliva.|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: V23-S293|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For KLK5 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||25818|
|Species Of This Entry||Human|
|Superfamily||peptidase S1 family|
|Alternative Names||Kallikrein 5|EC 3.4.21; EC 18.104.22.168; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme|
|Post Tranlational Modifications||Cleavage; Reduction; Glycosylation; Deacetylation; Methylation; Deglycosylation|
|Gene Location||19q13.41, on Chromasome 19, gene sequence: NC_000019.10 (50943299..50953038, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For KLK5 (Source: Uniprot.org, NCBI)
|Protein Function||May be involved in desquamation.|
|Tissue Specificity||Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.|
|Related Diseases||Malignant Neoplasms; Neoplasms; Malignant Neoplasm Of Ovary; Dermatologic Disorders; Ovarian Neoplasm; Malignant Neoplasm Of Breast; Dermatitis; Carcinoma; Dermatitis, Atopic; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Prostate; Ichthyosis Linearis Circumflexa; Mammary Neoplasms; Inflammation; Prostatic Neoplasms; Cell Invasion; Tissue Adhesions; Psoriasis; Rosacea; Skin Diseases, Genetic|
|Related Pathways||Pathogenesis; Localization; Proteolysis; Keratinocyte Differentiation; Reverse Transcription; Angiogenesis; Keratinization; Immune Response; Secretion; Cell Adhesion; Gene Silencing; Skin Development; Pigmentation; Cell Cycle Arrest; Embryo Implantation; Cell Cycle; Innate Immune Response; Cell-cell Adhesion; Histone Deacetylation; Cell Proliferation|
|Background||Kallikrein gene 5(KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.|
|Scientific References||PMID: 10514489 by Brattsand M., et al. Purification, molecular cloning, and expression of a human stratum corneum trypsin-like serine protease with possible function in desquamation.|
PMID: 10652563 by Yousef G.M., et al. Identification of novel human kallikrein-like genes on chromosome 19q13.3-q13.4.
Kit Components And Assay QC Data Details Of Human KLK5/Kallikrein 5 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0817-CAP||96-well plate precoated with anti-Human Kallikrein 5 antibody||1|
|EK0817-ST||lyophilized recombinant Human Kallikrein 5 standard||10ng/tube|
|EK0817-DA||biotinylated anti-Human Kallikrein 5 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0817 Human KLK5/Kallikrein 5 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human KLK5/Kallikrein 5 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human KLK5/Kallikrein 5 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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