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Product Info Summary
| SKU: | EK2236 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human Lefty ELISA Kit PicoKine®
SKU/Catalog Number
EK2236
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Lefty PicoKine ELISA Kit® (96 Tests). Quantitate Human Lefty in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 8 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Lefty ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2236)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: F78-P366
Sensitivity
<8 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2236 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2236 is reactive to LEFTY1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2236 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lefty-1
Left-right determination factor 1 is a protein that in humans is encoded by the LEFTY1 gene. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2236-CAP | Anti-Human LEFTY1 Pre-coated 96-well strip microplate | 1 |
| EK2236-ST | Human LEFTY1 Standard | 2 vials, 10 ng/tube |
| EK2236-DA | Human LEFTY1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.018 | 0.038 | 0.115 | 0.211 | 0.454 | 0.816 | 1.214 | 2.036 |
Data Example Images
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Human Lefty PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Lefty ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 28 | 56 | 449 | 25 | 58 | 354 |
| Standard deviation | 1.41 | 4.16 | 25.46 | 1.13 | 3.62 | 26.34 |
| CV(%) | 5% | 7.1% | 5.7% | 4.5% | 6.2% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Lefty-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 28 | 22 | 24 | 22 | 24 | 1.51 | 6.3% |
| Sample 2 | 59 | 33 | 58 | 38 | 47 | 3.48 | 7.4% |
| Sample 3 | 449 | 473 | 380 | 375 | 419 | 29.01 | 6.9% |
Protein Target Info & Infographic
Gene/Protein Information For LEFTY1 (Source: Uniprot.Org, NCBI)
Gene Name
LEFTY1
Full Name
Left-right determination factor 1
Weight
40.88kDa
Superfamily
TGF-beta family
Alternative Names
AI450052; EBAF; Leftb; lefty; Lefty1; Lefty-1; Lefty-B; Stra3; TGFB4 LEFTY1 LEFTB, LEFTYB left-right determination factor 1 left-right determination factor 1|left-right determination factor B|protein lefty-1|protein lefty-B
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LEFTY1, check out the LEFTY1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LEFTY1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Lefty ELISA Kit PicoKine® (EK2236)
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