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Product Info Summary
| SKU: | A05889 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Lefty LEFTY1 Antibody
SKU/Catalog Number
A05889
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Lefty LEFTY1 Antibody catalog # A05889. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lefty LEFTY1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05889)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human LEFTY1/LEFTY2. AA range:301-350
Reactive Species
A05889 is reactive to LEFTY1 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
40.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05889 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of HepG2-UV cells using Lefty Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
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Western Blot analysis of HEPG2-UV cells using Lefty Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-Lefty LEFTY1 Antibody (A05889)
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4 Customer Q&As for Anti-Lefty LEFTY1 Antibody
Question
My question regarding product A05889, anti-Lefty antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05889 anti-Lefty antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-17
Question
We are currently using anti-Lefty antibody A05889 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
The anti-Lefty antibody (A05889) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-17
Question
I see that the anti-Lefty antibody A05889 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-10-26
Question
Would anti-Lefty antibody A05889 work on horse WB with transverse colon?
S. Collins
Verified customer
Asked: 2013-09-19
Answer
Our lab technicians have not tested anti-Lefty antibody A05889 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Lefty antibody A05889 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse transverse colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-19
