Human Lipocalin-1 ELISA Kit PicoKine®

Lipocalin-1 ELISA kit for Human

Human Lipocalin-1 PicoKine ELISA Kit® (96 Tests). Quantitate Human Lipocalin-1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 60 pg/ml.

Product Info Summary

SKU: EK2229
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human Lipocalin-1 ELISA Kit PicoKine®

View all Lipocalin-1 ELISA kits

SKU/Catalog Number

EK2229

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Lipocalin-1 PicoKine ELISA Kit® (96 Tests). Quantitate Human Lipocalin-1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 60 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Lipocalin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2229)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: H19-D176

Sensitivity

<60 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2229 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2229 is reactive to LCN1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2229 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Lipocalin-1

Lipocalin-1 is a protein that in humans is encoded by the LCN1 gene. This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9.

Kit Components

Catalog Number Description Quantity
EK2229-CAP Anti-Human LCN1 Pre-coated 96-well strip microplate 1
EK2229-ST Human LCN1 Standard 2 vials, 10 ng/tube
EK2229-DA Human LCN1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)01252505001,0002,0004,0008,000
O.D.0.0880.1330.1990.3070.4840.8291.2541.911

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Lipocalin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)23946738911884273569
Standard deviation9.6112.24264.214.2531.38267.68
CV(%)4%2.6%6.8%7.6%7.4%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 123921323422122717.287.6%
Sample 246745147832643111.972.8%
Sample 338913425319626853299141.874.3%
*number of samples for each test n=16.

Gene/Protein Information For LCN1 (Source: Uniprot.Org, NCBI)

Gene Name

LCN1

Full Name

Lipocalin-1

Weight

19.25kDa

Superfamily

calycin superfamily

Alternative Names

LCN1; lipocalin 1 (tear prealbumin); lipocalin 1-like 2; Lipocalin1; Lipocalin-1; MGC71975; PMFA; Tear lipocalin; Tear Prealbumin; TLC; TPVon Ebner gland protein; VEG protein; VEGP; VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin) LCN1 PMFA, TLC, TP, VEGP lipocalin 1 lipocalin-1|VEG protein|Von Ebner gland protein|protein migrating faster than albumin|tear prealbumin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LCN1, check out the LCN1 Infographic

LCN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LCN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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