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Product Info Summary
| SKU: | EK2229 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human Lipocalin-1 ELISA Kit PicoKine®
View all Lipocalin-1 ELISA kits
SKU/Catalog Number
EK2229
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Lipocalin-1 PicoKine ELISA Kit® (96 Tests). Quantitate Human Lipocalin-1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 60 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Lipocalin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2229)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: H19-D176
Sensitivity
<60 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2229 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2229 is reactive to LCN1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2229 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lipocalin-1
Lipocalin-1 is a protein that in humans is encoded by the LCN1 gene. This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2229-CAP | Anti-Human LCN1 Pre-coated 96-well strip microplate | 1 |
| EK2229-ST | Human LCN1 Standard | 2 vials, 10 ng/tube |
| EK2229-DA | Human LCN1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 | 8,000 |
| O.D. | 0.088 | 0.133 | 0.199 | 0.307 | 0.484 | 0.829 | 1.254 | 1.911 |
Data Example Images
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Human Lipocalin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Lipocalin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 239 | 467 | 3891 | 188 | 427 | 3569 |
| Standard deviation | 9.61 | 12.24 | 264.2 | 14.25 | 31.38 | 267.68 |
| CV(%) | 4% | 2.6% | 6.8% | 7.6% | 7.4% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 239 | 213 | 234 | 221 | 227 | 17.28 | 7.6% |
| Sample 2 | 467 | 451 | 478 | 326 | 431 | 11.97 | 2.8% |
| Sample 3 | 3891 | 3425 | 3196 | 2685 | 3299 | 141.87 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For LCN1 (Source: Uniprot.Org, NCBI)
Gene Name
LCN1
Full Name
Lipocalin-1
Weight
19.25kDa
Superfamily
calycin superfamily
Alternative Names
LCN1; lipocalin 1 (tear prealbumin); lipocalin 1-like 2; Lipocalin1; Lipocalin-1; MGC71975; PMFA; Tear lipocalin; Tear Prealbumin; TLC; TPVon Ebner gland protein; VEG protein; VEGP; VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin) LCN1 PMFA, TLC, TP, VEGP lipocalin 1 lipocalin-1|VEG protein|Von Ebner gland protein|protein migrating faster than albumin|tear prealbumin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LCN1, check out the LCN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LCN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Lipocalin-1 ELISA Kit PicoKine® (EK2229)
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