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Product Info Summary
| SKU: | FEK2049 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CD206/CLEC13D PicoKine® Quick ELISA Kit
See all MMR/CD206/Mannose Receptor products
SKU/Catalog Number
FEK2049
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD206/CLEC13D PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD206/CLEC13D in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD206/CLEC13D PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK2049)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L19-K1383
Sensitivity
<50 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK2049 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK2049 is reactive to MRC1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
FEK2049 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMR/CD206/Mannose Receptor
The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). This gene is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK2049-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK2049-ST | Human MMR/CD206/Mannose Receptor Standard | 2 | 50 ng/tube |
| FEK2049-A | Human MRC1 Antibody A | 1 | 3ml |
| FEK2049-B | Human MRC1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.034 | 0.179 | 0.285 | 0.502 | 0.883 | 1.461 | 2.019 | 2.326 |
Data Example Images
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Human CD206 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1214 | 5652 | 25412 | 1333 | 6144 | 26014 |
| Standard deviation | 75.27 | 356.08 | 1296.01 | 85.31 | 423.94 | 1794.97 |
| CV(%) | 6.2% | 6.3% | 5.1% | 6.4% | 6.9% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMR/CD206/Mannose Receptor in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1214 | 1347 | 1449 | 1542 | 1388 | 68.01 | 4.9% |
| Sample 2 | 5652 | 6145 | 5784 | 5912 | 5873 | 340.65 | 5.8% |
| Sample 3 | 25412 | 26874 | 29748 | 25944 | 26995 | 1754.64 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For MRC1 (Source: Uniprot.Org, NCBI)
Gene Name
MRC1
Full Name
Macrophage mannose receptor 1
Weight
166.012kDa
Alternative Names
CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1 MRC1 CD206, CLEC13D, CLEC13DL, MMRL1, bA541I19.1, hMR, MRC1 mannose receptor C-type 1 macrophage mannose receptor 1|C-type lectin domain family 13 member D|human mannose receptor|macrophage mannose receptor 1-like protein 1|mannose receptor, C type 1-like 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MRC1, check out the MRC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MRC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD206/CLEC13D PicoKine® Quick ELISA Kit (FEK2049)
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