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Product Info Summary
| SKU: | EK2116 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human NCAN/Neurocan ELISA Kit PicoKine®
SKU/Catalog Number
EK2116
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human NCAN/Neurocan ELISA Kit PicoKine™ (96 Tests). Quantitate Human NCAN in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human NCAN/Neurocan ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2116)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: E23-C1321
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2116 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2116 is reactive to NCAN in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2116 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Neurocan
Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2116-CAP | Anti-Human NCAN Pre-coated 96-well strip microplate | 1 |
| EK2116-ST | Human NCAN Standard | 2 vials, 10 ng/tube |
| EK2116-DA | Human NCAN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.022 | 0.087 | 0.147 | 0.273 | 0.513 | 0.979 | 1.684 | 2.051 |
Data Example Images
Click image to see more details
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Neurocan, Dilution ratio of 1:1, concentration in serum and plasma is around 250 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human NCAN/Neurocan ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 83 | 221 | 972 | 75 | 236 | 860 |
| Standard deviation | 5.06 | 16.13 | 58.32 | 5.10 | 21.24 | 66.22 |
| CV (%) | 6.1% | 7.3% | 6% | 6.8% | 9% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Neurocan in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 83 | 91 | 88 | 85 | 87 | 4.52 | 5.2% |
| Sample 2 | 221 | 258 | 211 | 247 | 234 | 18.02 | 7.7% |
| Sample 3 | 972 | 965 | 933 | 954 | 956 | 80.30 | 8.4% |
Protein Target Info & Infographic
Gene/Protein Information For NCAN (Source: Uniprot.Org, NCBI)
Gene Name
NCAN
Full Name
Neurocan core protein
Weight
143.093kDa
Superfamily
aggrecan/versican proteoglycan family
Alternative Names
chondroitin sulfate proteoglycan 3 (neurocan); Chondroitin sulfate proteoglycan 3; CSPG3; CSPG3neurocan core protein; FLJ44681; NCAN; NEUR; neurocan proteoglycan; Neurocan NCAN CSPG3 neurocan neurocan core protein|chondroitin sulfate proteoglycan 3 (neurocan)|neurocan proteoglycan
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NCAN, check out the NCAN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NCAN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human NCAN/Neurocan ELISA Kit PicoKine® (EK2116)
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No publications found for EK2116
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human NCAN/Neurocan ELISA Kit PicoKine®
Question
Does EK2116 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human NCAN/Neurocan ELISA Kit PicoKine® (EK2116) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: Can Neurocan ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-12-22
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-12-22
Question
Q: how to analyze ELISA data? I have performed Neurocan level in serum.
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-05-07
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-04-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-04-13
Question
Q: how can I thaw whole blood sample for Neurocan ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Neurocan for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-09-21
Question
Q: is it okay to use heparin plasma as samples in Human Neurocan Picokine® ELISA Kit (Catalog # EK2116)?
P. Mangal
Verified customer
Asked: 2017-03-06
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Neurocan causing degradation of its protein structure. Neurocan may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Neurocan ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-03-06
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
K. Moore
Verified customer
Asked: 2015-03-04
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-03-04
