Human NUDT5 ELISA Kit PicoKine®

Product Name

Human NUDT5 ELISA Kit PicoKine®

View all ADP-Sugar Pyrophosphatase/NUDT5 ELISA kits

SKU/Catalog Number

EK2269

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human NUDT5 ELISA Kit PicoKine® (96 Tests). Quantitate Human NUDT5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 45 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human NUDT5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2269)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-F219

Sensitivity

<45 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2269 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2269 is reactive to NUDT5 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2269 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of ADP-Sugar Pyrophosphatase/NUDT5

ADP-sugar pyrophosphatase is an enzyme that in humans is encoded by the NUDT5 gene. This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors.

Kit Components

Catalog Number	Description	Quantity
EK2269-CAP	Anti-Human NUDT5 Pre-coated 96-well strip microplate	1
EK2269-ST	Human NUDT5 Standard	2 vials, 10 ng/tube
EK2269-DA	Human NUDT5 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	93.7	187.5	375	750	1,500	3,000	6,000
O.D.	0.031	0.067	0.136	0.385	0.541	0.781	1.163	1.878

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human NUDT5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ADP-Sugar Pyrophosphatase/NUDT5 in ELISA kits from four different production batches/lots.

Gene/Protein Information For NUDT5 (Source: Uniprot.Org, NCBI)

Alternative Names

EC 3.6.1.-; EC 3.6.1.13; hYSAH1; nucleoside diphosphate linked moiety X-type motif 5; Nucleoside diphosphate-linked moiety X motif 5; nudix (nucleoside diphosphate linked moiety X)-type motif 5; Nudix motif 5; NUDT5; YSA1; YSA1H; YSA1HADP-sugar pyrophosphatase NUDT5 YSA1, YSA1H, YSAH1, hNUDT5 nudix hydrolase 5 ADP-sugar pyrophosphatase|8-oxo-dGDP phosphatase|nuclear ATP-synthesis protein NUDIX5|nudix (nucleoside diphosphate linked moiety X)-type motif 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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