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Product Info Summary
| SKU: | EK2262 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human PCPE1 ELISA Kit PicoKine®
View all PCPE-1/PCOLCE ELISA kits
SKU/Catalog Number
EK2262
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PCPE1 ELISA Kit PicoKine® (96 Tests). Quantitate Human PCPE1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 300 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PCPE1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2262)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: M1-D449
Sensitivity
<300 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2262 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2262 is reactive to PCOLCE in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2262 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PCPE-1/PCOLCE
Procollagen C-endopeptidase enhancer 1 is an enzyme that in humans is encoded by the PCOLCE gene. Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2262-CAP | Anti-Human PCOLCE Pre-coated 96-well strip microplate | 1 |
| EK2262-ST | Human PCOLCE Standard | 2 vials, 40 ng/tube |
| EK2262-DA | Human PCOLCE Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 | 40,000 |
| O.D. | 0.015 | 0.193 | 0.236 | 0.375 | 0.698 | 0.934 | 1.473 | 2.056 |
Data Example Images
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Human PCPE1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PCPE1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 952 | 1335 | 18263 | 716 | 1356 | 10353 |
| Standard deviation | 53.03 | 97.05 | 1313.11 | 18.83 | 58.99 | 792 |
| CV(%) | 5.6% | 7.3% | 7.2% | 2.6% | 4.4% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PCPE-1/PCOLCE in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 952 | 1166 | 1130 | 693 | 985 | 50.15 | 5.1% |
| Sample 2 | 1335 | 2149 | 1773 | 1456 | 1668 | 39.37 | 2.4% |
| Sample 3 | 18623 | 16083 | 10251 | 14876 | 14868 | 793.96 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For PCOLCE (Source: Uniprot.Org, NCBI)
Gene Name
PCOLCE
Full Name
Procollagen C-endopeptidase enhancer 1
Weight
47.972kDa
Alternative Names
PCOLCE; PCPE; PCPE1; PCPE-1; PCPE1Type I procollagen COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer; Procollagen COOH-terminal proteinase enhancer 1; Procollagen C-proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; Type 1 procollagen C-proteinase enhancer protein PCOLCE PCPE, PCPE-1, PCPE1 procollagen C-endopeptidase enhancer procollagen C-endopeptidase enhancer 1|procollagen C-proteinase enhancer 1|procollagen COOH-terminal proteinase enhancer 1|procollagen, type 1, COOH-terminal proteinase enhancer|type 1 procollagen C-proteinase enhancer protein|type I procollagen COOH-terminal proteinase enhancer
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PCOLCE, check out the PCOLCE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PCOLCE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PCPE1 ELISA Kit PicoKine® (EK2262)
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