Mouse PCOLCE ELISA Kit PicoKine™

Mouse PCOLCE ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse PCOLCE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2106
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse PCOLCE ELISA Kit PicoKine™

See all PCPE-1/PCOLCE products

SKU/Catalog Number

EK2106

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse PCOLCE ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse PCOLCE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse PCOLCE ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2106)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-A468

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2106 is reactive to PCOLCE in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2106 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2106-CAP Anti-Mouse PCOLCE Pre-coated 96-well strip microplate 1
EK2106-ST Mouse PCOLCE Standard 2 vials, 10 ng/tube
EK2106-DA Mouse PCOLCE Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010,000
O.D.0.0150.0760.1340.2390.5090.9041.4572.265

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse PCOLCE ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Concentration (pg/ml)015631262512502500500010000
O.D.0.0530.1410.1990.3250.5020.9141.3771.992

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of PCPE-1/PCOLCE in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12422472462422442.270.9%
Sample 216441884178816011729113.046.5%
Sample 356315520514549735317268.195%
*number of samples for each test n=16.

Gene/Protein Information For PCOLCE (Source: Uniprot.Org, NCBI)

Uniprot ID

Q61398

Gene ID

18542

Gene Name

PCOLCE

Full Name

Procollagen C-endopeptidase enhancer 1

Weight

50.168kDa

Alternative Names

PCOLCE; PCPE; PCPE1; PCPE-1; PCPE1Type I procollagen COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer; Procollagen COOH-terminal proteinase enhancer 1; Procollagen C-proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; Type 1 procollagen C-proteinase enhancer protein Pcolce|Ast, Astt, Astt-2, Astt2, P14, PCPE-1|procollagen C-endopeptidase enhancer protein|procollagen C-endopeptidase enhancer 1|astroglial cell transcript 2|procollagen C-proteinase enhancer 1|procollagen C-proteinase enhancer protein|procollagen COOH-terminal proteinase enhancer 1|type 1 procollagen C-proteinase enhancer protein|type I procollagen COOH-terminal proteinase enhancer

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PCOLCE, check out the PCOLCE Infographic

PCOLCE infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PCOLCE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Mouse PCOLCE ELISA Kit PicoKine™

Question

Q: which procedure should I follow in order to thaw whole blood sample for PCPE-1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-11-17

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PCPE-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-11-17

Question

Q: if the enzyme conjugated PCPE-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PCPE-1 antigen?

H. Evans

Verified customer

Asked: 2020-02-24

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-02-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-07-18

Question

Q: how to proceed with the analysis of ELISA data? I measured PCPE-1 level in plasma.

Verified Customer

Verified customer

Asked: 2019-04-18

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-04-18

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement PCPE-1 using the EK2106 Mouse PCPE-1 Picokine® ELISA Kit?

S. Evans

Verified customer

Asked: 2018-04-08

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-04-08

Question

Q: is it okay to use citrate plasma as samples in Mouse PCPE-1 Picokine® ELISA Kit (Catalog # EK2106)?

Verified Customer

Verified customer

Asked: 2017-05-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of PCPE-1 causing degradation of its protein structure. PCPE-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the PCPE-1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-05-22

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

C. Smith

Verified customer

Asked: 2015-08-29

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2015-08-29

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

J. Robinson

Verified customer

Asked: 2014-08-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2014-08-03

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