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Product Info Summary
| SKU: | EK2258 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human PNP ELISA Kit PicoKine®
View all Purine Nucleoside Phosphorylase/PNP ELISA kits
SKU/Catalog Number
EK2258
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PNP ELISA Kit PicoKine® (96 Tests). Quantitate Human PNP in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PNP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2258)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-S289
Sensitivity
<15 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2258 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2258 is reactive to PNP in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2258 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Purine Nucleoside Phosphorylase/PNP
Purine nucleoside phosphorylase, PNP, PNPase or inosine phosphorylase (EC 2.4.2.1) is an enzyme that in humans is encoded by the NP gene. This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2258-CAP | Anti-Human PNP Pre-coated 96-well strip microplate | 1 |
| EK2258-ST | Human PNP Standard | 2 vials, 10 ng/tube |
| EK2258-DA | Human PNP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.035 | 0.108 | 0.166 | 0.265 | 0.475 | 1.060 | 1.455 | 2.061 |
Data Example Images
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Human PNP PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PNP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 36 | 117 | 639 | 48 | 83 | 827 |
| Standard deviation | 2.45 | 4.25 | 29.52 | 3.27 | 5.07 | 29.77 |
| CV(%) | 6.8% | 3.6% | 4.6% | 6.8% | 6.1% | 3.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Purine Nucleoside Phosphorylase/PNP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 54 | 39 | 56 | 46 | 2.38 | 5.1% |
| Sample 2 | 117 | 96 | 91 | 114 | 105 | 4.92 | 4.7% |
| Sample 3 | 639 | 729 | 746 | 580 | 674 | 39.27 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For PNP (Source: Uniprot.Org, NCBI)
Gene Name
PNP
Full Name
Purine nucleoside phosphorylase
Weight
32.118kDa
Superfamily
PNP/MTAP phosphorylase family
Alternative Names
EC 2.4.2.1; FLJ94043; FLJ97288; Inosine phosphorylase; MGC117396; MGC125915; MGC125916; NPFLJ97312; nucleoside phosphorylase; PNP; PRO1837; PUNP; purine nucleoside phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase PNP NP, PRO1837, PUNP purine nucleoside phosphorylase purine nucleoside phosphorylase|HEL-S-156an|epididymis secretory sperm binding protein Li 156an|inosine phosphorylase|inosine-guanosine phosphorylase|purine-nucleoside:orthophosphate ribosyltransferase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PNP, check out the PNP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PNP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PNP ELISA Kit PicoKine® (EK2258)
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