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Product Info Summary
| SKU: | EK2158 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human sCD2 ELISA Kit PicoKine®
SKU/Catalog Number
EK2158
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human sCD2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human sCD2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2158)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: HEK293; Immunogen sequence: K25-D209
Sensitivity
<5 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2158 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2158 is reactive to CD2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2158 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD2
CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2158-CAP | Anti-Human CD2 Pre-coated 96-well strip microplate | 1 |
| EK2158-ST | Human CD2 Standard | 2 vials, 10 ng/tube |
| EK2158-DA | Human CD2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
| O.D. | 0.039 | 0.150 | 0.246 | 0.465 | 0.697 | 1.088 | 1.627 | 2.023 |
Data Example Images
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Human sCD2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human sCD2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 134 | 815 | 3563 | 141 | 789 | 3651 |
| Standard deviation | 8.44 | 43.2 | 167.46 | 10.01 | 48.92 | 200.81 |
| CV(%) | 6.3% | 5.3% | 4.7% | 7.1% | 6.2% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 134 | 157 | 155 | 149 | 149 | 9.67 | 6.5% |
| Sample 2 | 815 | 800 | 742 | 766 | 781 | 36.7 | 4.7% |
| Sample 3 | 3563 | 3565 | 3941 | 3594 | 3666 | 150.3 | 4.1% |
Protein Target Info & Infographic
Gene/Protein Information For CD2 (Source: Uniprot.Org, NCBI)
Gene Name
CD2
Full Name
T-cell surface antigen CD2
Weight
39.448kDa
Alternative Names
CD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5 CD2 LFA-2, SRBC, T11 CD2 molecule T-cell surface antigen CD2|CD2 antigen (p50), sheep red blood cell receptor|LFA-3 receptor|T-cell surface antigen T11/Leu-5|erythrocyte receptor|lymphocyte-function antigen-2|rosette receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD2, check out the CD2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human sCD2 ELISA Kit PicoKine® (EK2158)
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