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Product Info Summary
| SKU: | EK2136 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human ZAG/AZGP1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2136
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ZAG/AZGP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human AZGP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ZAG/AZGP1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2136)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q21-S298
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2136 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2136 is reactive to AZGP1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2136 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ZAG
Zinc-alpha-2-glycoprotein is a protein that in humans is encoded by the AZGP1 gene. Alpha-2-glycoprotein, also known as AZGP1, belongs to the MHC class I family. It can be detected in body fluids such as serum, sweat, and seminal and breast cyst fluids. It has been shown that alpha-2-glycoprotein can stimulate lipolysis by adipocytes in vivo and in vitro. Thus it is believed that alpha-2-glycoprotein plays an important role in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and it also regulates melanin production by normal and malignant melanocytes. Alpha-2-glycoprotein is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2136-CAP | Anti-Human AZGP1 Pre-coated 96-well strip microplate | 1 |
| EK2136-ST | Human AZGP1 Standard | 2 vials, 10 ng/tube |
| EK2136-DA | Human AZGP1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.023 | 0.134 | 0.291 | 0.502 | 0.958 | 1.364 | 1.651 | 2.095 |
Data Example Images
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Human ZAG/AZGP1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ZAG, Dilution ratio of 1:10000, concentration in serum and plasma is 3.7-24.7 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ZAG/AZGP1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 452 | 2653 | 8848 | 445 | 2841 | 9012 |
| Standard deviation | 24.86 | 124.69 | 637.06 | 32.93 | 159.1 | 720.93 |
| CV(%) | 5.5% | 4.7% | 7.2% | 7.4% | 5.6% | 8.0% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ZAG in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 452 | 501 | 486 | 477 | 479 | 21.56 | 4.5% |
| Sample 2 | 2653 | 2854 | 2963 | 2832 | 2826 | 135.62 | 4.8% |
| Sample 3 | 8848 | 8512 | 9041 | 8614 | 8754 | 533.98 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For AZGP1 (Source: Uniprot.Org, NCBI)
Gene Name
AZGP1
Full Name
Zinc-alpha-2-glycoprotein
Weight
34.259kDa
Superfamily
MHC class I family
Alternative Names
alpha-2-glycoprotein 1, zinc; alpha-2-glycoprotein 1, zinc-binding; AZGP1; ZA2G; ZAG; ZAGzn-alpha-2-GP; zinc; zinc-alpha-2-glycoprotein; zn-alpha-2-glycoprotein; ZNGP1 AZGP1 ZA2G, ZAG alpha-2-glycoprotein 1, zinc-binding zinc-alpha-2-glycoprotein|Alpha-2-glycoprotein, zinc|Zn-alpha2-glycoprotein|testicular tissue protein Li 227|zn-alpha-2-GP|zn-alpha-2-glycoprotein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on AZGP1, check out the AZGP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for AZGP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ZAG/AZGP1 ELISA Kit PicoKine® (EK2136)
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