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Product Info Summary
| SKU: | LS152404 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of immunoglobulin superfamily, member 11 (IGSF11), transcript variant 1 |
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Product info
Product Name
IGSF11 (NM_152538) Human Over-expression Lysate
SKU/Catalog Number
LS152404
Size
100 µg
Description
Transient overexpression lysate of immunoglobulin superfamily, member 11 (IGSF11), transcript variant 1
Storage & Handling
Cite This Product
IGSF11 (NM_152538) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS152404)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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IGSF11 Human Over-expression Lysates NM_152538.
Western blot validation of overexpression lysate (LS152404) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IGSF11 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for IGSF11 (Source: Uniprot.org, NCBI)
Gene Name
IGSF11
Full Name
Immunoglobulin superfamily member 11
Weight
46.12kDa
Alternative Names
Brain and testis-specific immunoglobulin superfamily protein; BTIGSF; BT-IgSF; cancer/testis antigen 119; CT119; CXADR like 1; CXADRL1; IGSF11; Igsf13; immunoglobulin superfamily member 11; immunoglobulin superfamily, member 11; MGC35227; V-set and immunoglobulin domain containing 3; V-set and immunoglobulin domain-containing protein 3; VSIG3; VSIG3brain and testis-specific immunoglobin superfamily protein IGSF11 BT-IgSF, CT119, CXADRL1, Igsf13, VSIG3 immunoglobulin superfamily member 11 immunoglobulin superfamily member 11|CXADR like 1|V-set and immunoglobulin domain containing 3|V-set and immunoglobulin domain-containing protein 3|brain and testis-specific immunoglobin superfamily protein|brain and testis-specific immunoglobulin superfamily protein|cancer/testis antigen 119
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IGSF11, check out the IGSF11 Infographic
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In this infographic, you will see the following information for IGSF11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IGSF11 (NM_152538) Human Over-expression Lysate (LS152404)
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No publications found for LS152404
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