Product Info Summary
SKU: | LS125476 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of INO80 complex subunit C (INO80C), transcript variant 1 |
Product info
Product Name
INO80C (NM_001098817) Human Over-expression Lysate
SKU/Catalog Number
LS125476
Size
100 µg
Description
Transient overexpression lysate of INO80 complex subunit C (INO80C), transcript variant 1
Storage & Handling
Cite This Product
INO80C (NM_001098817) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS125476)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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INO80C Human Over-expression Lysates NM_001098817.
Western blot validation of overexpression lysate (LS125476) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with INO80C (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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INO80C Human Over-expression Lysates NM_194281.
Western blot validation of overexpression lysate (LS125476) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with INO80C (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for INO80C (Source: Uniprot.org, NCBI)
Gene Name
INO80C
Full Name
INO80 complex subunit C
Weight
20.643kDa
Alternative Names
C18orf37; chromosome 18 open reading frame 37; FLJ38183; hIes6; IES6 homolog; IES6; INO80 complex subunit C INO80C C18orf37, IES6, hIes6 INO80 complex subunit C INO80 complex subunit C|IES6 homolog
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on INO80C, check out the INO80C Infographic
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In this infographic, you will see the following information for INO80C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For INO80C (NM_001098817) Human Over-expression Lysate (LS125476)
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