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Product Info Summary
| SKU: | LS081930 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of kinesin family member 18A (KIF18A) |
Product info
Product Name
KIF18A (NM_031217) Human Over-expression Lysate
SKU/Catalog Number
LS081930
Size
100 µg
Description
Transient overexpression lysate of kinesin family member 18A (KIF18A)
Storage & Handling
Cite This Product
KIF18A (NM_031217) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081930)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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KIF18A Human Over-expression Lysates NM_031217.
Western blot validation of overexpression lysate (LS081930) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KIF18A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for KIF18A (Source: Uniprot.org, NCBI)
Gene Name
KIF18A
Full Name
Kinesin-like protein KIF18A
Weight
102.281kDa
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
DKFZP434G2226; kinesin family member 18A; kinesin-like protein KIF18A; Marrow stromal KIF18A; MS-KIF18A KIF18A MS-KIF18A, PPP1R99 kinesin family member 18A kinesin-like protein KIF18A|protein phosphatase 1, regulatory subunit 99
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on KIF18A, check out the KIF18A Infographic
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In this infographic, you will see the following information for KIF18A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For KIF18A (NM_031217) Human Over-expression Lysate (LS081930)
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No publications found for LS081930
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