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Product Info Summary
| SKU: | LS051621 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of Kruppel-like factor 13 (KLF13) |
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Product info
Product Name
KLF13 (NM_015995) Human Over-expression Lysate
SKU/Catalog Number
LS051621
Size
100 µg
Description
Transient overexpression lysate of Kruppel-like factor 13 (KLF13)
Storage & Handling
Cite This Product
KLF13 (NM_015995) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS051621)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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KLF13 Human Over-expression Lysates NM_015995.
Western blot validation of overexpression lysate (LS051621) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KLF13 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Klf13 (Source: Uniprot.org, NCBI)
Gene Name
Klf13
Full Name
Krueppel-like factor 13
Weight
31.136kDa
Superfamily
Sp1 C2H2-type zinc-finger protein family
Alternative Names
basic transcription element binding protein 3; Basic transcription element-binding protein 3; BTEB3FKLF2; BTE-binding protein 3; Krueppel-like factor 13; Kruppel-like factor 13; Novel Sp1-like zinc finger transcription factor 1; NSLP1RFLAT1; RANTES factor of late activated T lymphocytes-1; RANTES factor of late activated T-lymphocytes 1; RFLAT-1FKLF-2; Sp1 like zinc finger transcription factor; Transcription factor BTEB3; Transcription factor NSLP1 Klf13|0610043C13Rik, 9430029L20Rik, Bteb3, FKLF-, FKLF-2, FKLF2, Klf1, N, NSLP1, RFL, RFLA, RFLAT-1, RFLAT1|Kruppel-like factor 13|Krueppel-like factor 13|BTE-binding protein 3|C2-H2 zinc finger protein|RANTES factor of late activated T-lymphocytes 1|basic transcription element-binding protein 3|erythroid transcription factor FKLF-2|transcription factor BTEB3
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Klf13, check out the Klf13 Infographic
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In this infographic, you will see the following information for Klf13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For KLF13 (NM_015995) Human Over-expression Lysate (LS051621)
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