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Product Info Summary
| SKU: | LS010660 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ladybird homeobox 1 (LBX1) |
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Product info
Product Name
LBX1 (NM_006562) Human Over-expression Lysate
SKU/Catalog Number
LS010660
Size
100 µg
Description
Transient overexpression lysate of ladybird homeobox 1 (LBX1)
Storage & Handling
Cite This Product
LBX1 (NM_006562) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010660)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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LBX1 Human Over-expression Lysates NM_006562.
Western blot validation of overexpression lysate (LS010660) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with LBX1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for LBX1 (Source: Uniprot.org, NCBI)
Gene Name
LBX1
Full Name
Transcription factor LBX1
Weight
30.221kDa
Alternative Names
homeobox; HPX6; HPX-6; ladybird homeobox 1; ladybird homeobox homolog 1 (Drosophila); ladybird homeobox homolog 1; Ladybird homeobox protein homolog 1; LBX1Hlady bird-like homeobox; transcription factor LBX1; transcription factor similar to D. melanogaster homeodomain protein lady birdlate LBX1 HPX-6, HPX6H, homeobox, LBX1 ladybird homeobox 1 transcription factor LBX1|lady bird-like homeobox|ladybird homeobox homolog 1|ladybird homeobox protein homolog 1|transcription factor similar to D. melanogaster homeodomain protein lady bird late
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on LBX1, check out the LBX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LBX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For LBX1 (NM_006562) Human Over-expression Lysate (LS010660)
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No publications found for LS010660
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