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Product Info Summary
| SKU: | LS084648 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of late cornified envelope 3D (LCE3D) |
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Product info
Product Name
LCE3D (NM_032563) Human Over-expression Lysate
SKU/Catalog Number
LS084648
Size
100 µg
Description
Transient overexpression lysate of late cornified envelope 3D (LCE3D)
Storage & Handling
Cite This Product
LCE3D (NM_032563) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084648)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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LCE3D Human Over-expression Lysates NM_032563.
Western blot validation of overexpression lysate (LS084648) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with LCE3D (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for LCE3D (Source: Uniprot.org, NCBI)
Gene Name
LCE3D
Full Name
Late cornified envelope protein 3D
Weight
9.444kDa
Superfamily
LCE family
Alternative Names
Late cornified envelope protein 3D LCE3D LEP16, SPRL6A, SPRL6B late cornified envelope 3D late cornified envelope protein 3D|late envelope protein 16|small proline rich-like (epidermal differentiation complex) 6A|small proline rich-like (epidermal differentiation complex) 6B|small proline-rich-like epidermal differentiation complex protein 6A|small proline-rich-like epidermal differentiation complex protein 6B
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on LCE3D, check out the LCE3D Infographic
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In this infographic, you will see the following information for LCE3D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For LCE3D (NM_032563) Human Over-expression Lysate (LS084648)
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No publications found for LS084648
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