Product Info Summary
SKU: | LS001241 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of leukotriene B4 receptor (LTB4R), transcript variant 1 |
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Product info
Product Name
Leukotriene B4 Receptor (LTB4R) (NM_181657) Human Over-expression Lysate
See all Leukotriene B4 R1 products
SKU/Catalog Number
LS001241
Size
100 µg
Description
Transient overexpression lysate of leukotriene B4 receptor (LTB4R), transcript variant 1
Storage & Handling
Cite This Product
Leukotriene B4 Receptor (LTB4R) (NM_181657) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001241)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Leukotriene B4 Receptor (LTB4R) Human Over-expression Lysates NM_001143919.
Western blot validation of overexpression lysate (LS001241) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with LTB4R (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
Leukotriene B4 Receptor (LTB4R) Human Over-expression Lysates NM_181657.
Western blot validation of overexpression lysate (LS001241) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with LTB4R (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for LTB4R (Source: Uniprot.org, NCBI)
Gene Name
LTB4R
Full Name
Leukotriene B4 receptor 1
Weight
37.557kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
BLT1; chemokine receptor-like 1; CMKRL1; Leukotriene B4 R1; leukotriene B4 receptor; Leukotriene B4R1; LTB4R; LTB4R1; LTBR1; P2Y purinoceptor 7; purinergic receptor P2Y, G-protein coupled, 7 LTB4R BLT1, BLTR, CMKRL1, GPR161, LTBR1, P2RY7, P2Y7, LTB4R leukotriene B4 receptor leukotriene B4 receptor 1|G protein-coupled receptor 16|LTB4-R 1|LTB4-R1|P2Y purinoceptor 7|chemoattractant receptor-like 1|chemokine receptor-like 1|purinergic receptor P2Y, G-protein coupled, 7
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on LTB4R, check out the LTB4R Infographic
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In this infographic, you will see the following information for LTB4R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Leukotriene B4 Receptor (LTB4R) (NM_181657) Human Over-expression Lysate (LS001241)
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