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Product Info Summary
| SKU: | LS011027 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2 (LILRA2), transcript variant 2 |
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Product info
Product Name
LILRA2 (NM_006866) Human Over-expression Lysate
See all ILT1/CD85h/LILRA2 products
SKU/Catalog Number
LS011027
Size
100 µg
Description
Transient overexpression lysate of leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2 (LILRA2), transcript variant 2
Storage & Handling
Cite This Product
LILRA2 (NM_006866) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS011027)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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LILRA2 Human Over-expression Lysates NM_006866.
Western blot validation of overexpression lysate (LS011027) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with LILRA2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for LILRA2 (Source: Uniprot.org, NCBI)
Gene Name
LILRA2
Full Name
Leukocyte immunoglobulin-like receptor subfamily A member 2
Weight
52.992kDa
Alternative Names
CD85 antigen-like family member H; CD85h antigen; CD85h; ILT1; ILT-1; ILT1CD85H; Leukocyte immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily A member 2 soluble; leukocyte immunoglobulin-like receptor subfamily A member 2; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2; LILRA2; LIR7; LIR7LIR-7Immunoglobulin-like transcript 1 LILRA2 CD85H, ILT1, LIR-7, LIR7 leukocyte immunoglobulin like receptor A2 leukocyte immunoglobulin-like receptor subfamily A member 2|CD85 antigen-like family member H|immunoglobulin-like transcript 1|leucocyte Ig-like receptor A2|leukocyte immunoglobulin-like receptor 7|leukocyte immunoglobulin-like receptor subfamily A member 2 soluble|leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on LILRA2, check out the LILRA2 Infographic
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In this infographic, you will see the following information for LILRA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For LILRA2 (NM_006866) Human Over-expression Lysate (LS011027)
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No publications found for LS011027
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