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Product Info Summary
| SKU: | LS023608 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of makorin ring finger protein 1 (MKRN1), transcript variant 1 |
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Product info
Product Name
MKRN1 (NM_013446) Human Over-expression Lysate
SKU/Catalog Number
LS023608
Size
100 µg
Description
Transient overexpression lysate of makorin ring finger protein 1 (MKRN1), transcript variant 1
Storage & Handling
Cite This Product
MKRN1 (NM_013446) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023608)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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MKRN1 Human Over-expression Lysates NM_001145125.
Western blot validation of overexpression lysate (LS023608) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MKRN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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MKRN1 Human Over-expression Lysates NM_013446.
Western blot validation of overexpression lysate (LS023608) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MKRN1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for MKRN1 (Source: Uniprot.org, NCBI)
Gene Name
MKRN1
Full Name
E3 ubiquitin-protein ligase makorin-1
Weight
53.349kDa
Alternative Names
E3 ubiquitin-protein ligase makorin-1; EC 6.3.2.-; FLJ21334; makorin ring finger protein 1; RNF61RING finger protein 61 MKRN1 RNF61 makorin ring finger protein 1 E3 ubiquitin-protein ligase makorin-1|RING finger protein 61|RING-type E3 ubiquitin transferase makorin-1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on MKRN1, check out the MKRN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MKRN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For MKRN1 (NM_013446) Human Over-expression Lysate (LS023608)
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No publications found for LS023608
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