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Product Info Summary
| SKU: | LS064210 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19) |
Product info
Product Name
MMS19 (NM_022362) Human Over-expression Lysate
See all MMS19 like protein products
SKU/Catalog Number
LS064210
Size
100 µg
Description
Transient overexpression lysate of MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19)
Storage & Handling
Cite This Product
MMS19 (NM_022362) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS064210)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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MMS19 Human Over-expression Lysates NM_022362.
Western blot validation of overexpression lysate (LS064210) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MMS19 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for MMS19 (Source: Uniprot.org, NCBI)
Gene Name
MMS19
Full Name
MMS19 nucleotide excision repair protein homolog
Weight
113.29kDa
Superfamily
MET18/MMS19 family
Alternative Names
FLJ34167; MET18 homolog; MET18; MMS19 nucleotide excision repair homolog (S. cerevisiae); S. cerevisiae) MMS19 CIAO4, MET18L, hMMS19, MMS19 MMS19 homolog, cytosolic iron-sulfur assembly component MMS19 nucleotide excision repair protein homolog|MET18 homolog|MMS19 cytosolic iron-sulfur assembly component|MMS19 nucleotide excision repair homolog|MMS19-like (MET18 homolog, S. cerevisiae)|MMS19-like protein|homolog of yeast MMS19
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on MMS19, check out the MMS19 Infographic
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In this infographic, you will see the following information for MMS19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For MMS19 (NM_022362) Human Over-expression Lysate (LS064210)
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