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Product Info Summary
| SKU: | EK2091 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse CD99L2 ELISA Kit PicoKine®
SKU/Catalog Number
EK2091
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CD99L2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cd99l2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CD99L2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2091)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: D26-G161
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2091 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2091 is reactive to CD99L2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2091 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD99-L2
CD99 antigen-like protein 2 is a protein that in humans is encoded by the CD99L2 gene. It is mapped to X; X A7.3. This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2091-CAP | Anti-Mouse CD99L2 Pre-coated 96-well strip microplate | 1 |
| EK2091-ST | Mouse CD99L2 Standard | 2 vials, 20 ng/tube |
| EK2091-DA | Mouse CD99L2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.062 | 0.144 | 0.218 | 0.378 | 0.612 | 0.939 | 1.446 | 2.059 |
Data Example Images
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Mouse CD99L2 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD99L2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 467 | 1310 | 7832 | 529 | 2102 | 8406 |
| Standard deviation | 19.15 | 56.33 | 360.27 | 30.68 | 145.04 | 605.23 |
| CV (%) | 4.1% | 4.3% | 4.6% | 5.8% | 6.9% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD99-L2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 467 | 427 | 448 | 472 | 453 | 17.72 | 3.9% |
| Sample 2 | 1310 | 1396 | 1545 | 1357 | 1402 | 87.99 | 6.2% |
| Sample 3 | 7832 | 7176 | 7216 | 8274 | 7624 | 456.32 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For CD99L2 (Source: Uniprot.Org, NCBI)
Gene Name
CD99L2
Full Name
CD99 antigen-like protein 2
Weight
27.986kDa
Superfamily
CD99 family
Alternative Names
CD99 antigen; CD99 antigen-like 2; CD99 antigen-like protein 2; CD99 molecule-like 2; CD99B; CD99L2; CD99-L2; DKFZp761H2024; MIC2 like 1; MIC2L1; MIC2-like protein 1 CD99L2 CD99B, MIC2L1 CD99 molecule like 2 CD99 antigen-like protein 2|MIC2 like 1|MIC2-like protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD99L2, check out the CD99L2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD99L2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CD99L2 ELISA Kit PicoKine® (EK2091)
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No publications found for EK2091
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Mouse CD99L2 ELISA Kit PicoKine®
Question
Q: is it okay to use EDTA plasma as samples in Mouse CD99-L2 Picokine® ELISA Kit (Catalog # EK2091)?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CD99-L2 causing degradation of its protein structure. CD99-L2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD99-L2 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-01-22
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CD99-L2 using the EK2091 Mouse CD99-L2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-01-04
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-01-04
Question
Q: What is the optimal O.D. value for CD99-L2 ELISA kit? I used your CD99-L2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD99-L2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD99-L2 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of CD99-L2 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-06-14
Question
Q: Can CD99-L2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Jackson
Verified customer
Asked: 2018-09-25
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-25
Question
Q: how can I thaw whole blood sample for CD99-L2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-08-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD99-L2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-08-11
