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Product Info Summary
| SKU: | EK2010 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine®
View all YM1/Chitinase 3-like 3 ELISA kits
SKU/Catalog Number
EK2010
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Chil3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2010)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Y22-Y398
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2010 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2010 is reactive to Chil3 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2010 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of YM1/Chitinase 3-like 3
Chitinase 3-like 3, also known as ECF-L and Ym1, is a secreted 377 amino acid lectin that binds heparin and GlcN oligomers. This gene is mapped to 3 F2.2; 3 46.49 Cm. CHI3L3 is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins, but lacks enzymatic activity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2010-CAP | Anti-Mouse Chil3 Pre-coated 96-well strip microplate | 1 |
| EK2010-ST | Mouse Chil3 Standard | 2 vials, 10 ng/tube |
| EK2010-DA | Mouse Chil3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.030 | 0.076 | 0.123 | 0.221 | 0.403 | 0.754 | 1.313 | 2.035 |
Data Example Images
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Mouse CHI3L3 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect YM1/Chitinase 3-like 3, Dilution ratio of 1:20, concentration in serum and plasma is around 50 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 327 | 1000 | 4032 | 308 | 1073 | 3654 |
| Standard deviation | 13.73 | 67 | 221.76 | 18.78 | 83.69 | 204.62 |
| CV (%) | 4.2% | 6.7% | 5.5% | 6.1% | 7.8% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of YM1/Chitinase 3-like 3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 327 | 332 | 292 | 319 | 317 | 15.43 | 4.8% |
| Sample 2 | 1000 | 1134 | 1146 | 1166 | 1111 | 65.38 | 5.8% |
| Sample 3 | 4032 | 3785 | 4495 | 4279 | 4147 | 265.89 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For Chil3 (Source: Uniprot.Org, NCBI)
Gene Name
Chil3
Full Name
Chitinase-like protein 3
Weight
44.458kDa
Superfamily
glycosyl hydrolase 18 family
Alternative Names
AI505981; CHI3L3; Chitinase 3 like 3; Chitinase 3-like 3; ECF-L; YM1 Chil3|AI505981, Chi3, Chi3l3, ECF-L, Ym1|chitinase-like 3|chitinase-like protein 3|beta-N-acetylhexosaminidase Ym1|eosinophil chemotactic cytokine|eosinophil chemotactic factor-L|secreted protein Ym1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Chil3, check out the Chil3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Chil3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine® (EK2010)
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No publications found for EK2010
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine®
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-01-12
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-01-12
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Y. Thompson
Verified customer
Asked: 2019-09-15
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-09-15
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-08
Question
Q: is it okay to use EDTA plasma as samples in Mouse YM1 Picokine® ELISA Kit (Catalog # EK2010)?
M. Martinez
Verified customer
Asked: 2018-08-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of YM1 causing degradation of its protein structure. YM1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the YM1 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-08-15
Question
Q: how do I analyze ELISA data? I have obtained YM1 level in serum.
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-06-15
Question
Q: can you recommend the dilution ratio of serum samples for detection of YM1 in Mouse serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the YM1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-11
